ALCOHOL AND THE CELLS

  • Thomas, Andrew (PI)
  • Baserga, Renato Luigi (PI)
  • Chance, Britton (PI)
  • Farber, John (PI)
  • Ellingson, John (PI)
  • Taraschi, Theodore (PI)
  • Stubbs, Christopher (PI)
  • Hoek, Jan (PI)
  • Rubin, Emanuel (PI)
  • RUBIN, RAPHAEL (PI)
  • Iswrael, Yedy (PI)
  • Cavarrubias, Maneel (PI)
  • Ponnappa, B. (PI)

Project Details

Description

Increasing evidence suggests that the deleterious effects of alcohol abuse
are related to changes at the cellular level. We, therefore, have
assembled a group of biochemists, biophysicists and pathologists to explore
the central theme of "Alcohol and the Cell". We propose to study the
alterations in structure and function produced by ethanol and/or
acetaldehyde in vitro, and of chronic ethanol feeding, in cells from
various organs and subcellular constitutents, e.g., membranes,
mitochondria, sarcoplasmic and endoplasmic reticulum, synaptosomes, etc.,
using the techniques of biophysics, biochemistry and quantitative
histochemistry. Such techniques include fluorescence polarization,
fluorescence lifetime measurements, electron paramagnetic resonance,
nuclear magnetic resonance, X-ray and neutron diffraction, particle
electrophoresis and electron microscopic immunocytochemistry. We intend to
study the physical properties of membranes, alcohol-dependent necrosis,
mitochondrial membrane assembly and the response to hormones in the liver
and other tissues. Metabolic studies will include 31P NMR investigations
of changes in nucleotide and metabolite levels in isolated cells and
perfused organs, quantitative histochemistry of individual cells localized
in the lobules of the liver and studies of collagen production by
individual cells, using electron microscopic immune histochemistry. It is
likely that the initial effects of ethanol are similar in all cells, and
that the patterns of injury and adaptation reflect cellular
specialization. It is therefore reasonable to hope that the elucidation of
alterations at the cellular level will provide a key to the understanding
of various ethanol-related diseases.
StatusFinished
Effective start/end date7/1/8611/30/03

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

ASJC

  • Medicine(all)

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