Project Details

Description

Abstract This project will develop and verify a microtissue engineered bioreactor platform to expand bone marrow cancer stem cells and create xenograft models using frozen MDS patient marrow cells. The project specific aims are: (1) Specific Aim 1: Microfluidic bioreactor for human HSC expansion from a low volume marrow sample; (2) Specific Aim 2: Genetically engineered scaffolds to recreate a MDS microenvironment ex vivo; and (3) Specific Aim 3: Ex vivo differentiation testing of MDS stem cells to predict leukemic transformation. The deliverable of the project will be a qualified bioreactor culture system and protocol to expand rare cells, such as pre-leukemic stem cells, from a small volume bone marrow biopsy tissue sample for use in prospective clinical trials.
StatusFinished
Effective start/end date8/1/115/31/22

Funding

  • National Institute of Biomedical Imaging and Bioengineering: $559,849.00
  • National Institute of Biomedical Imaging and Bioengineering: $544,399.00
  • National Institute of Biomedical Imaging and Bioengineering: $681,859.00

ASJC

  • Biotechnology

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