Project Details
Description
DESCRIPTION (Adapted from the applicant's specific aims): Transcription
factors play important roles in controlling development in response to
cellular or external signals in all organisms. A subfamily of b-zipper
proteins has been identified from Arabidopsis, resulted from a previous
investigation of the nuclear factor which binds to a plant viral promoter
element. Arabidopsis have about seven members of this group of b-zipper
factors and their functional roles are currently unknown. In this project,
methods will be developed for a gene disruption protocol to knockout each
of the individual genes and to reveal functional roles through examining
the phenotypic defects of those gene disruption lines. During this funding
period, two different members of this gene family, TGA2 and TGA3, will be
chosen as targets for the gene disruption study since genomic clones for
these loci have already been obtained. The lab have shown the feasibility
of gene disruption in plant cell culture system, and is in the process of
optimizing and adapting into whole plant level. The success of this study
will not only elucidate the roles for these two members of this particular
b-zipper family of plant transcription factor, it will also open the way
for targeted gene disruption analysis of other interesting genes in higher
plants. This is a high risk but high reward research effort.
Status | Finished |
---|---|
Effective start/end date | 8/1/96 → 7/31/99 |
Funding
- National Institute of General Medical Sciences
ASJC
- Genetics
- Molecular Biology
Fingerprint
Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.