Project Details

Description

The common objective of these projects is to exploit and test the
various clues that have recently come to light on the functions of
gangliosides in the nervous system. Thus, our recent discovery
that gangliosides are present at relatively high levels in synaptic
vesicles of Torpedo (and possibly other species) provides an
opportunity to investigate their role in neurosecretion. As a
first step we shall determine the structures and distributional
pattern of molecular species in this organelle and compare them to
the structures and distributional pattern of pre-synaptic membrane
from the same source. The neuritogenic effect which exogenous
gangliosides assert on cultured neurons and certain neuronally-
derived cells lines will be studied from the standpoint of
mechanism and also by comparing the ganglioside patterns and
synthesis potential of responding vs non-responding cells. We
shall attempt to determine which pool of exogenous gangliosides
causes the effect. These studies will probe the role of
differentiation-associated gangliosides in the normal
differentiation of neurons. We shall continue our study of
gangliosides of the peripheral nervous system, testing our
hypothesis that ganglio-type structures are common to all neurons
of the CNS and PNS. This system will also be used to study
ganglioside role(s) in regeneration. The two ganglioside transfer
proteins we have isolated from calf brain will be further purified
and characterized as to glycolipid specificity. Their cellular and
subcellular localization within brain will be studied by use of
antibodies. We shall explore their use as research tools for
modifying glycolipid composition of membranes. This will include
examination of possible heterogeneity of composition in such
membranes from different brain regions and different neuronal
types. Using cell culture systems, we shall determine whether
growth cone membranes have unique gangliosides not characteristics
of the remainder of the differentiating neuron.Description
StatusFinished
Effective start/end date12/1/7811/30/94

Funding

  • National Institutes of Health
  • National Institutes of Health: $148,384.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $368,113.00
  • National Institutes of Health: $211,343.00
  • National Institutes of Health

ASJC

  • Medicine(all)
  • Neuroscience(all)

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