β-endorphin modulation of interferon-γ, perforin and granzyme B levels in splenic NK cells: Effects of ethanol

Madhavi Dokur; Ping Chen Cui; Juan P. Advis; Dipak K. Sarkar

doi:https://doi.org/10.1016/j.jneuroim.2005.03.015

β-endorphin modulation of interferon-γ, perforin and granzyme B levels in splenic NK cells: Effects of ethanol

Madhavi Dokur, Ping Chen Cui, Juan P. Advis, Dipak K. Sarkar

Rutgers, The State University

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The effects of ethanol and beta-endorphin (β-EP) on productions of cytolytic factors granzyme B, perforin and IFN-γ in splenic rat NK cells were determined. Intracranial administration of β-EP increased protein and mRNA levels of cytolytic factors in NK cells. Chronic ethanol feeding reduced the basal and β-EP-induced levels of cytolytic factors in NK cells. In vitro treatment of β-EP on NK cells increased the levels of perforin, granzyme B and IFN-γ and their mRNA transcripts, whereas ethanol pre-treatment prevented β-EP effects on cytolytic factors in these cells. These results suggest that β-EP and ethanol interact to regulate NK cell functions.

Original language	English (US)
Pages (from-to)	29-38
Number of pages	10
Journal	Journal of Neuroimmunology
Volume	166
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2005.03.015
State	Published - Sep 2005

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

Keywords

Cytotoxic factors
Enriched natural killer cells
Hypothalamus
Splenocytes
β-endorphin

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https://doi.org/10.1016/j.jneuroim.2005.03.015

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title = "β-endorphin modulation of interferon-γ, perforin and granzyme B levels in splenic NK cells: Effects of ethanol",

abstract = "The effects of ethanol and beta-endorphin (β-EP) on productions of cytolytic factors granzyme B, perforin and IFN-γ in splenic rat NK cells were determined. Intracranial administration of β-EP increased protein and mRNA levels of cytolytic factors in NK cells. Chronic ethanol feeding reduced the basal and β-EP-induced levels of cytolytic factors in NK cells. In vitro treatment of β-EP on NK cells increased the levels of perforin, granzyme B and IFN-γ and their mRNA transcripts, whereas ethanol pre-treatment prevented β-EP effects on cytolytic factors in these cells. These results suggest that β-EP and ethanol interact to regulate NK cell functions.",

keywords = "Cytotoxic factors, Enriched natural killer cells, Hypothalamus, Splenocytes, β-endorphin",

author = "Madhavi Dokur and Cui, {Ping Chen} and Advis, {Juan P.} and Sarkar, {Dipak K.}",

note = "Funding Information: This investigation was supported by National Institutes of Health Grant AA12642.",

year = "2005",

month = sep,

doi = "https://doi.org/10.1016/j.jneuroim.2005.03.015",

language = "English (US)",

volume = "166",

pages = "29--38",

journal = "Journal of Neuroimmunology",

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T1 - β-endorphin modulation of interferon-γ, perforin and granzyme B levels in splenic NK cells

T2 - Effects of ethanol

AU - Dokur, Madhavi

AU - Cui, Ping Chen

AU - Advis, Juan P.

AU - Sarkar, Dipak K.

N1 - Funding Information: This investigation was supported by National Institutes of Health Grant AA12642.

PY - 2005/9

Y1 - 2005/9

N2 - The effects of ethanol and beta-endorphin (β-EP) on productions of cytolytic factors granzyme B, perforin and IFN-γ in splenic rat NK cells were determined. Intracranial administration of β-EP increased protein and mRNA levels of cytolytic factors in NK cells. Chronic ethanol feeding reduced the basal and β-EP-induced levels of cytolytic factors in NK cells. In vitro treatment of β-EP on NK cells increased the levels of perforin, granzyme B and IFN-γ and their mRNA transcripts, whereas ethanol pre-treatment prevented β-EP effects on cytolytic factors in these cells. These results suggest that β-EP and ethanol interact to regulate NK cell functions.

AB - The effects of ethanol and beta-endorphin (β-EP) on productions of cytolytic factors granzyme B, perforin and IFN-γ in splenic rat NK cells were determined. Intracranial administration of β-EP increased protein and mRNA levels of cytolytic factors in NK cells. Chronic ethanol feeding reduced the basal and β-EP-induced levels of cytolytic factors in NK cells. In vitro treatment of β-EP on NK cells increased the levels of perforin, granzyme B and IFN-γ and their mRNA transcripts, whereas ethanol pre-treatment prevented β-EP effects on cytolytic factors in these cells. These results suggest that β-EP and ethanol interact to regulate NK cell functions.

KW - Cytotoxic factors

KW - Enriched natural killer cells

KW - Hypothalamus

KW - Splenocytes

KW - β-endorphin

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DO - https://doi.org/10.1016/j.jneuroim.2005.03.015

M3 - Article

C2 - 16005984

VL - 166

SP - 29

EP - 38

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

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IS - 1-2

ER -

β-endorphin modulation of interferon-γ, perforin and granzyme B levels in splenic NK cells: Effects of ethanol

Abstract

ASJC Scopus subject areas

Keywords

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