2,2-Diphenyl-1-picrylhydrazyl radical-scavenging active components from Polygonum multiflorum Thunb.

Yong Chen, Mingfu Wang, Robert T. Rosen, Chi Tang Ho

Research output: Contribution to journalArticlepeer-review

243 Scopus citations


An activity-directed fractionation and purification process was used to identify the antioxidative components of Polygonum multiflorum Thunb. (PM). Dried root of PM was extracted with 95% ethanol and then separated into water, ethyl acetate, and hexane fractions. Among these only the ethyl acetate phase showed strong antioxidant activity by the 2,2-diphenyl-1- picrylhydrazyl (DPPH) test when compared with water and hexane phases. The ethyl acetate fraction was then subjected to separation and purification using silica gel column chromatography and Sephadex LH-20 chromatography. Three compounds showing strong antioxidant activity were identified by spectral methods (1H NMR, 13C NMR, and MS) and by comparison with authentic samples to be gallic acid, catechin, and 2,3,5,4'- tetrahydroxystilbene 2-O-β-D-glucopyranoside.

Original languageEnglish (US)
Pages (from-to)2226-2228
Number of pages3
JournalJournal of agricultural and food chemistry
Issue number6
StatePublished - Jun 1999

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)


  • 2,3,5,4'- tetrahydroxystilbene 2-O-β-D-glucopyranoside
  • Antioxidant activity
  • Free radical
  • Polygonum multiflorum Thunb.


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