A gene from Pseudomonas syringae pv. glycinea with homology to avirulence gene D from P. s. pv. tomato but devoid of the avirulence phenotype.

Donald Kobayashi, S. J. Tamaki, D. J. Trollinger, S. Gold, N. T. Keen

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A gene was cloned from Pseudomonas syringae pv. glycinea that hybridized to avirulence gene D (avrD), previously cloned from P. s. pv. tomato. Unlike avrD, the hypersensitive response (HR) was not elicited when the P. s. pv. glycinea gene was reintroduced into P. s. pv. glycinea race 4 on a broad host range plasmid and the bacteria were inoculated into soybean leaves. DNA sequence data disclosed that the P. s. pv. glycinea homologue of avrD encoded a protein containing 86% identical amino acids to avrD, with substitutions distributed throughout the protein. Two ORFs immediately downstream from the avrD homologue were more similar in P. s. pv. tomato and P. s. pv. glycinea, with 98 and 99% identical amino acids. Expression of the wildtype P. s. pv. glycinea gene and recombinant genes constructed between the P. s. pv. tomato avrD gene and its P. s. pv. glycinea homologue in both Escherichia coli and P. s. pv. glycinea indicated that the P. s. pv. glycinea gene product was formed less efficiently or was less stable than was the P. s. pv. tomato protein encoded by avrD. The data indicated that the P. s. pv. glycinea homologue represents a recessive allele of the P. s. pv. tomato avrD gene which has been modified by mutation such that it does not lead to an avirulence phenotype on the normal host plant, soybean.

Original languageEnglish (US)
Pages (from-to)103-111
Number of pages9
JournalMolecular plant-microbe interactions : MPMI
Volume3
Issue number2
DOIs
StatePublished - Jan 1 1990
Externally publishedYes

Fingerprint

Pseudomonas syringae pv. glycinea
Pseudomonas syringae
Lycopersicon esculentum
tomatoes
Phenotype
phenotype
Genes
genes
Soybeans
soybeans
Amino Acids
Proteins
amino acids

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Physiology

Cite this

@article{c03b506ba5c44c76ad78dc08fe595d49,
title = "A gene from Pseudomonas syringae pv. glycinea with homology to avirulence gene D from P. s. pv. tomato but devoid of the avirulence phenotype.",
abstract = "A gene was cloned from Pseudomonas syringae pv. glycinea that hybridized to avirulence gene D (avrD), previously cloned from P. s. pv. tomato. Unlike avrD, the hypersensitive response (HR) was not elicited when the P. s. pv. glycinea gene was reintroduced into P. s. pv. glycinea race 4 on a broad host range plasmid and the bacteria were inoculated into soybean leaves. DNA sequence data disclosed that the P. s. pv. glycinea homologue of avrD encoded a protein containing 86{\%} identical amino acids to avrD, with substitutions distributed throughout the protein. Two ORFs immediately downstream from the avrD homologue were more similar in P. s. pv. tomato and P. s. pv. glycinea, with 98 and 99{\%} identical amino acids. Expression of the wildtype P. s. pv. glycinea gene and recombinant genes constructed between the P. s. pv. tomato avrD gene and its P. s. pv. glycinea homologue in both Escherichia coli and P. s. pv. glycinea indicated that the P. s. pv. glycinea gene product was formed less efficiently or was less stable than was the P. s. pv. tomato protein encoded by avrD. The data indicated that the P. s. pv. glycinea homologue represents a recessive allele of the P. s. pv. tomato avrD gene which has been modified by mutation such that it does not lead to an avirulence phenotype on the normal host plant, soybean.",
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A gene from Pseudomonas syringae pv. glycinea with homology to avirulence gene D from P. s. pv. tomato but devoid of the avirulence phenotype. / Kobayashi, Donald; Tamaki, S. J.; Trollinger, D. J.; Gold, S.; Keen, N. T.

In: Molecular plant-microbe interactions : MPMI, Vol. 3, No. 2, 01.01.1990, p. 103-111.

Research output: Contribution to journalArticle

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AU - Kobayashi, Donald

AU - Tamaki, S. J.

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AU - Keen, N. T.

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