A mixed hemagglutination test for binding of glycosylated cytochemical markers

TY - JOUR

T1 - A mixed hemagglutination test for binding of glycosylated cytochemical markers

AU - Mureşan, Virgil

AU - Constantinescu, Marius C.

N1 - Funding Information: ’ This work was supported by the Ministry of Education (Romania) and by NIH Grant HL-26343 awarded to Nicolae Simionescu and Maya Simionescu. * Present address: “Dr. Gh. Marinescu” Neurosurgery Clinic, Bucharest, Romania. 3 Abbreviations used: RCA. Ricinus communis agglutinin 120; BSA, bovine serum albumin; HGM, hog gastric mucin glycopeptides; F, fetuin; ORS, orosomucoid; AF, asialofetuin; AORS, asialoorosomucoid; GCM. glycosylated cytochemical markers; DPBS. Dulbecco’s phosphate-buffered saline; hRBC, human red blood cells: sRBC, sheep red blood cells; RCA-sRBC, Ricinus communis agglutinin l20-coupled sheep red blood cells: BSA-sRBC, bovine serum albumin-coupled sheep red blood cells: FITC, fluorescein isothiocyanate.

PY - 1985/8/1

Y1 - 1985/8/1

N2 - A sensitive hemagglutination test which uses simultaneously erythrocytes from two different species was developed to test the ability of glycosylated cytochemical markers to interact with membrane-bound lectins. The interaction between galactose-terminated glycoconjugates and Ricinus communis agglutinin 120 was chosen for exemplification. The lectin was covalently attached to the surface of sheep red blood cells, thus rendering them highly agglutinating for human erythrocytes. Small amounts of a large variety of terminal galactose-containing molecules inhibited the hemagglutination. The assay proved to be particularly useful for testing colloidal gold-adsorbed glycoconjugates of low protein concentration.

AB - A sensitive hemagglutination test which uses simultaneously erythrocytes from two different species was developed to test the ability of glycosylated cytochemical markers to interact with membrane-bound lectins. The interaction between galactose-terminated glycoconjugates and Ricinus communis agglutinin 120 was chosen for exemplification. The lectin was covalently attached to the surface of sheep red blood cells, thus rendering them highly agglutinating for human erythrocytes. Small amounts of a large variety of terminal galactose-containing molecules inhibited the hemagglutination. The assay proved to be particularly useful for testing colloidal gold-adsorbed glycoconjugates of low protein concentration.

KW - glutaraldehyde coupling

KW - glycoprotein-gold complexes

KW - glycosylated cytochemical markers

KW - hemagglutination

KW - immobilized lectins

KW - membrane lectins

UR - http://www.scopus.com/inward/record.url?scp=0021857644&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021857644&partnerID=8YFLogxK

U2 - https://doi.org/10.1016/0003-2697(85)90259-3

DO - https://doi.org/10.1016/0003-2697(85)90259-3

M3 - Article

C2 - 4061825

VL - 148

SP - 503

EP - 509

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 2

ER -