A modular cross-linking approach for exploring protein interactions

Michelle Trester-Zedlitz, Katsuhiko Kamada, Stephen Burley, David Fenyö, Brian T. Chait, Tom W. Muir

Research output: Contribution to conferencePaper

Abstract

The protein-protein interaction was described using cross-linking reagents and mass spectrometry. This approach was applied for the study of cross-linking between the components of the heterodimeric protein complex, Negative Cofactor 2 (NC2). Five crosslinking reagents were synthesized using solid phase chemistry, by availing the chemical versatility of the mercaptopropionamide-PEGA resin-linker. It was found that there is an intermolecular interaction between the N-terminal regions of NC2 α and β, as well as three intra-molecular and several dead-end cross-links.

Original languageEnglish (US)
Pages273-274
Number of pages2
StatePublished - Dec 1 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

Fingerprint

Cross-Linking Reagents
Proteins
Mass spectrometry
poly(acryloyl-bis(aminopropyl)polyethylene glycol)

All Science Journal Classification (ASJC) codes

  • Spectroscopy

Cite this

Trester-Zedlitz, M., Kamada, K., Burley, S., Fenyö, D., Chait, B. T., & Muir, T. W. (2002). A modular cross-linking approach for exploring protein interactions. 273-274. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.
Trester-Zedlitz, Michelle ; Kamada, Katsuhiko ; Burley, Stephen ; Fenyö, David ; Chait, Brian T. ; Muir, Tom W. / A modular cross-linking approach for exploring protein interactions. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.2 p.
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Trester-Zedlitz, M, Kamada, K, Burley, S, Fenyö, D, Chait, BT & Muir, TW 2002, 'A modular cross-linking approach for exploring protein interactions' Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02 - 6/6/02, pp. 273-274.

A modular cross-linking approach for exploring protein interactions. / Trester-Zedlitz, Michelle; Kamada, Katsuhiko; Burley, Stephen; Fenyö, David; Chait, Brian T.; Muir, Tom W.

2002. 273-274 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conferencePaper

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AU - Trester-Zedlitz, Michelle

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AU - Muir, Tom W.

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Trester-Zedlitz M, Kamada K, Burley S, Fenyö D, Chait BT, Muir TW. A modular cross-linking approach for exploring protein interactions. 2002. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.