A morphometric examination of type II alveolar epithelial cells in normal and isolated‐perfused dog lungs

David Defouw, Peter B. Berendsen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The volume densities of type II alveolar cell cytoplasmic organelles and alveolar surface densities were estimated by established stereologic procedures. The morphometric measurements were obtained from normal dog lungs (in situ) and isolated dog lungs perfused for 30‐minute, 1‐hour, and 2‐hour periods. The type II cell lamellar body volume densities and the alveolar surface densities progressively decreased as the times of perfusion were increased. The volume densities of the granular and agranular endoplasmic reticulum progressively increased during the periods of perfusion. These morphometric parameters from lungs in situ and isolated lungs suggest that changes occur in pulmonary surfactant synthesis and activity during perfusion. It is further postulated that progressive increases in the rates of surfactant removal and/or inactivation during perfusion may contribute to spontaneous edema in lungs isolated for periods exceeding two hours. The morphologic and physiologic integrity of isolated perfused lung preparations, widely used as models of lungs in vivo, in situ requires further evaluation.

Original languageEnglish (US)
Pages (from-to)139-147
Number of pages9
JournalAmerican Journal of Anatomy
Volume150
Issue number1
DOIs
StatePublished - Jan 1 1977

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Alveolar Epithelial Cells
Dogs
Lung
Perfusion
Pulmonary Surfactants
Smooth Endoplasmic Reticulum
Rough Endoplasmic Reticulum
Surface-Active Agents
Organelles
Edema

All Science Journal Classification (ASJC) codes

  • Anatomy

Cite this

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A morphometric examination of type II alveolar epithelial cells in normal and isolated‐perfused dog lungs. / Defouw, David; Berendsen, Peter B.

In: American Journal of Anatomy, Vol. 150, No. 1, 01.01.1977, p. 139-147.

Research output: Contribution to journalArticle

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