A pre-loading method of evaluating gap junctional communication by fluorescent dye transfer

G. S. Goldberg, J. F. Bechberger, C. C.G. Naus

Research output: Contribution to journalArticlepeer-review

200 Scopus citations

Abstract

We describe a simple method for evaluating gap junctional communication (GJC) between cells in culture. The procedure involves pre-loading cells with two fluorescent dyes: calcein and Dil. Calcein is able to pass through gap junctions, while Dil is not. These pre-loaded cells are then plated with unlabeled cells. The number of cells receiving calcein from each pre-loaded cell can then be quantified after the cells settle on the plate. Potent and reversible inhibitors of GJC can be used in this system to evaluate dye transfer within a given period of time.

Original languageEnglish (US)
Pages (from-to)490-497
Number of pages8
JournalBioTechniques
Volume18
Issue number3
StatePublished - Jan 1 1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biotechnology

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