We describe a simple method for evaluating gap junctional communication (GJC) between cells in culture. The procedure involves pre-loading cells with two fluorescent dyes: calcein and Dil. Calcein is able to pass through gap junctions, while Dil is not. These pre-loaded cells are then plated with unlabeled cells. The number of cells receiving calcein from each pre-loaded cell can then be quantified after the cells settle on the plate. Potent and reversible inhibitors of GJC can be used in this system to evaluate dye transfer within a given period of time.
|Original language||English (US)|
|Number of pages||8|
|State||Published - Jan 1 1995|
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)