Keyphrases
Mammalian Cells
100%
Clustered Regularly Interspaced Short Palindromic repeats/Cas9
100%
Vector Construction
100%
Donor Vector
100%
Detailed Protocol
50%
Gene of Interest
30%
Mammalian Cell Lines
30%
Specific Genes
20%
Knock-in Mouse Model
20%
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)
20%
Genome Editing
20%
Custom-made
20%
Epitope Tag
20%
Neomycin Phosphotransferase II (npt II)
20%
Gene Truncation
20%
Gibson Assembly
20%
Wide-ranging
10%
Commercially Available
10%
Adaptive Immune Response
10%
Genetic Investigation
10%
Guide RNA (gRNA)
10%
Polymerase Chain Reaction
10%
Screening Method
10%
Homologous Recombination
10%
Expression Vector
10%
Western Blot Analysis
10%
RNA-based
10%
Prokaryotes
10%
Foreign DNA
10%
CRISPR/Cas9 Genome Editing
10%
Gene Deletion
10%
G418
10%
Cassette
10%
Vector Design
10%
Sanger Sequencing
10%
Associated Sequence
10%
Step Method
10%
Geneticin
10%
Gene Modification
10%
Neomycin Resistance
10%
Cas9D10A Nickase
10%
Medicine and Dentistry
Mammalian Cell
100%
Clustered Regularly Interspaced Short Palindromic Repeat
100%
Cas9
100%
Spine
100%
Cell Line
42%
Genome Editing
42%
Protein Tag
28%
G418
28%
Polymerase Chain Reaction
14%
Screening
14%
Guide RNA
14%
Adaptive Immunity
14%
Expression Vector
14%
Western Blot
14%
Homologous Recombination
14%
Gene Deletion
14%
Neomycin
14%
Sanger Sequencing
14%
Kanamycin Kinase
14%
Biochemistry, Genetics and Molecular Biology
Mammalian Cell
100%
CRISPR
100%
Cas9
100%
Genome Editing
42%
Neomycin
28%
G418
28%
Protein Tag
28%
Genetics
14%
Phosphotransferase
14%
Polymerase Chain Reaction
14%
RNA
14%
Expression Vector
14%
Homologous Recombination
14%
Western Blot
14%
Prokaryote
14%
Adaptive Immunity
14%
Screening
14%
Gene Deletion
14%
Sanger Sequencing
14%
Guide RNA
14%