A route to RNA with an alkylating group at the 5′-triphosphate residue

M. A. Grachev, A. A. Mustaev, S. I. Oshevski

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Reaction of ATP with N,N,N′-tris(2-chloroethyl),N′(p-formylphenyl)propylenediamine-1,3 (abbreviation Cl3R) afforded a γ-ester of ATP (abbreviation CLRpppA)-the product of alkylation by an aliphatic nitrogen mustard residue of Cl3R. The alkylating activity of the aromatic nitrogen mustard residue of ClRpppA is suppressed by the electron-acceptor effect of the p-fomyl group. ClRpppA is a substrate of RNA-polymerase, and affords RNA with ClRpppA-residues at the 5′-termini.

Original languageEnglish (US)
Pages (from-to)3413-3426
Number of pages14
JournalNucleic acids research
Volume8
Issue number15
DOIs
StatePublished - Aug 11 1980
Externally publishedYes

Fingerprint

Mechlorethamine
RNA
Adenosine Triphosphate
Alkylation
DNA-Directed RNA Polymerases
Esters
Electrons
ClRpppA
triphosphoric acid

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Grachev, M. A. ; Mustaev, A. A. ; Oshevski, S. I. / A route to RNA with an alkylating group at the 5′-triphosphate residue. In: Nucleic acids research. 1980 ; Vol. 8, No. 15. pp. 3413-3426.
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A route to RNA with an alkylating group at the 5′-triphosphate residue. / Grachev, M. A.; Mustaev, A. A.; Oshevski, S. I.

In: Nucleic acids research, Vol. 8, No. 15, 11.08.1980, p. 3413-3426.

Research output: Contribution to journalArticle

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AU - Mustaev, A. A.

AU - Oshevski, S. I.

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