Abstract
A simple method to select disomic (N + 1) strains that should be applicable for almost any chromosome in Saccharomyces cerevisiae is presented. A diploid heterozygous for a KanMX knock-out mutation in an essential gene is sporulated and viable geneticin (G418)-resistant colonies selected. Disomic products of a missegregation or non-disjunction event containing a copy of both the wild-type essential gene and its complementary KanMX knock-out allele make up most of the viable colonies. This method has been used to isolate disomic haploids for a variety of chromosomes. It is appropriately named MARV (for missegregation-associated restoration of viability) and is easily adaptable to virtually any strain.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 321-326 |
| Number of pages | 6 |
| Journal | Yeast |
| Volume | 25 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 2008 |
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biochemistry
- Applied Microbiology and Biotechnology
- Genetics
Keywords
- Aneuploid
- Yeast