A theoretical formalism for aggregation of peroxidized lipids and plasma membrane stability during photolysis

N. A. Busch, M. L. Yarmush, Mehmet Toner

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The objective of this investigation was to examine, from a theoretical perspective, the mechanism underlying the lysis of plasma membranes by photoinduced, chemically mediated damage such as is found in photolysis. Toward this end, a model is presented which relates the membrane lifetime to the thermodynamic parameters of the membrane components based upon the kinetic theory of aggregate formation. The formalism includes a standard birth/death process for the formation of damaged membrane components (i.e., peroxidized lipids) as well as a terminating condensation process for the formation of aggregates of peroxidized plasma membrane lipids. Our theory predicts that 1) the membrane lifetime is inversely correlated with predicted rate of membrane damage; 2) an upper limit on the duration of membrane damage exists, above which the mean and variance of the membrane lifetime is independent of further membrane damage; and 3) both the mean and variance of the time of membrane lifetime distribution are correlated with the number of sites that may be damaged to form a single membrane defect. The model provides a framework to optimize the lysis of cell membranes by photodynamic therapy.

Original languageEnglish (US)
Pages (from-to)2956-2970
Number of pages15
JournalBiophysical journal
Volume75
Issue number6
DOIs
StatePublished - Dec 1998

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Photolysis
Cell Membrane
Lipids
Membranes
Photochemotherapy
Membrane Lipids
Thermodynamics
Parturition

All Science Journal Classification (ASJC) codes

  • Biophysics

Cite this

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A theoretical formalism for aggregation of peroxidized lipids and plasma membrane stability during photolysis. / Busch, N. A.; Yarmush, M. L.; Toner, Mehmet.

In: Biophysical journal, Vol. 75, No. 6, 12.1998, p. 2956-2970.

Research output: Contribution to journalArticle

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