Abstract
The objective of this investigation was to examine, from a theoretical perspective, the mechanism underlying the lysis of plasma membranes by photoinduced, chemically mediated damage such as is found in photolysis. Toward this end, a model is presented which relates the membrane lifetime to the thermodynamic parameters of the membrane components based upon the kinetic theory of aggregate formation. The formalism includes a standard birth/death process for the formation of damaged membrane components (i.e., peroxidized lipids) as well as a terminating condensation process for the formation of aggregates of peroxidized plasma membrane lipids. Our theory predicts that 1) the membrane lifetime is inversely correlated with predicted rate of membrane damage; 2) an upper limit on the duration of membrane damage exists, above which the mean and variance of the membrane lifetime is independent of further membrane damage; and 3) both the mean and variance of the time of membrane lifetime distribution are correlated with the number of sites that may be damaged to form a single membrane defect. The model provides a framework to optimize the lysis of cell membranes by photodynamic therapy.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2956-2970 |
| Number of pages | 15 |
| Journal | Biophysical journal |
| Volume | 75 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 1998 |
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All Science Journal Classification (ASJC) codes
- Biophysics
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A theoretical formalism for aggregation of peroxidized lipids and plasma membrane stability during photolysis. / Busch, N. A.; Yarmush, M. L.; Toner, Mehmet.
In: Biophysical journal, Vol. 75, No. 6, 12.1998, p. 2956-2970.Research output: Contribution to journal › Article
TY - JOUR
T1 - A theoretical formalism for aggregation of peroxidized lipids and plasma membrane stability during photolysis
AU - Busch, N. A.
AU - Yarmush, M. L.
AU - Toner, Mehmet
PY - 1998/12
Y1 - 1998/12
N2 - The objective of this investigation was to examine, from a theoretical perspective, the mechanism underlying the lysis of plasma membranes by photoinduced, chemically mediated damage such as is found in photolysis. Toward this end, a model is presented which relates the membrane lifetime to the thermodynamic parameters of the membrane components based upon the kinetic theory of aggregate formation. The formalism includes a standard birth/death process for the formation of damaged membrane components (i.e., peroxidized lipids) as well as a terminating condensation process for the formation of aggregates of peroxidized plasma membrane lipids. Our theory predicts that 1) the membrane lifetime is inversely correlated with predicted rate of membrane damage; 2) an upper limit on the duration of membrane damage exists, above which the mean and variance of the membrane lifetime is independent of further membrane damage; and 3) both the mean and variance of the time of membrane lifetime distribution are correlated with the number of sites that may be damaged to form a single membrane defect. The model provides a framework to optimize the lysis of cell membranes by photodynamic therapy.
AB - The objective of this investigation was to examine, from a theoretical perspective, the mechanism underlying the lysis of plasma membranes by photoinduced, chemically mediated damage such as is found in photolysis. Toward this end, a model is presented which relates the membrane lifetime to the thermodynamic parameters of the membrane components based upon the kinetic theory of aggregate formation. The formalism includes a standard birth/death process for the formation of damaged membrane components (i.e., peroxidized lipids) as well as a terminating condensation process for the formation of aggregates of peroxidized plasma membrane lipids. Our theory predicts that 1) the membrane lifetime is inversely correlated with predicted rate of membrane damage; 2) an upper limit on the duration of membrane damage exists, above which the mean and variance of the membrane lifetime is independent of further membrane damage; and 3) both the mean and variance of the time of membrane lifetime distribution are correlated with the number of sites that may be damaged to form a single membrane defect. The model provides a framework to optimize the lysis of cell membranes by photodynamic therapy.
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U2 - https://doi.org/10.1016/S0006-3495(98)77737-9
DO - https://doi.org/10.1016/S0006-3495(98)77737-9
M3 - Article
C2 - 9826616
VL - 75
SP - 2956
EP - 2970
JO - Biophysical Journal
JF - Biophysical Journal
SN - 0006-3495
IS - 6
ER -