Accumulation of p53 in a mutant cell line defective in the ubiquitin pathway

Dondapati R. Chowdary, James Dermody, Krishna K. Jha, Harvey L. Ozer

Research output: Contribution to journalArticle

262 Citations (Scopus)

Abstract

The wild-type p53 gene product plays an important role in the control of cell proliferation, differentiation, and survival. Altered function is frequently associated with changes in p53 stability. We have studied the role of the ubiquitination pathway in the degradation of p53, utilizing a temperature-sensitive mutant, ts20, derived from the mouse cell line BALB/c 3T3. We found that wild-type p53 accumulates markedly because of decreased breakdown when cells are shifted to the restrictive temperature. Introduction of sequences encoding the human ubiquitin-activating enzyme E1 corrects the temperature sensitivity defect in ts20 and prevents accumulation of p53. The data therefore strongly indicate that wild-type p53 is degraded intracellularly by the ubiquitin-mediated proteolytic pathway.

Original languageEnglish (US)
Pages (from-to)1997-2003
Number of pages7
JournalMolecular and cellular biology
Volume14
Issue number3
DOIs
StatePublished - Jan 1 1994

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Ubiquitin
Cell Line
Temperature
Ubiquitination
p53 Genes
Cell Differentiation
Cell Survival
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Chowdary, Dondapati R. ; Dermody, James ; Jha, Krishna K. ; Ozer, Harvey L. / Accumulation of p53 in a mutant cell line defective in the ubiquitin pathway. In: Molecular and cellular biology. 1994 ; Vol. 14, No. 3. pp. 1997-2003.
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Accumulation of p53 in a mutant cell line defective in the ubiquitin pathway. / Chowdary, Dondapati R.; Dermody, James; Jha, Krishna K.; Ozer, Harvey L.

In: Molecular and cellular biology, Vol. 14, No. 3, 01.01.1994, p. 1997-2003.

Research output: Contribution to journalArticle

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