TY - JOUR
T1 - Accumulation of silver(I) ion and diamine silver complex by Aeromonas SH10 biomass
AU - Zhang, Haoran
AU - Li, Qingbiao
AU - Wang, Huixuan
AU - Sun, Daohua
AU - Lu, Yinghua
AU - He, Ning
N1 - Funding Information: Acknowledgment This work is part of the project (20376076) supported by National Natural Science Foundation of China. The authors thank Analysis and Testing Center of Xiamen University for the help of SEM and TEM analysis in this study.
PY - 2007/10
Y1 - 2007/10
N2 - The biomass of Aeromonas SH10 was proven to strongly absorb Ag+ and [Ag(NH3)2]+. The maximum uptake of [Ag(NH3)2]+ was 0.23 g(Ag) g-1(cell dry weight), higher than that of Ag+. Fourier transform infrared spectroscopy spectra analysis indicated that some organic groups, such as amide and ionized carboxyl in the cell wall, played an important role in the process of biosorption. After SH10 cells were suspended in the aqueous solution of [Ag(NH3)2]+ under 60°C for more than 12 h, [Ag(NH3)2]+ was reduced to Ag(0), which was demonstrated by the characteristic absorbance peak of elemental silver nanoparticle in UV-VIS spectrum. Scanning electron microscopy and transmission electron microscopy observation showed that nanoparticles were formed on the cell wall after reduction. These particles were then confirmed to be elemental silver crystal by energy dispersive X-ray spectroscopy, X-ray diffraction, and UV-VIS analysis. This study demonstrated the potential use of Aeromonas SH10 in silver-containing wastewater treatment due to its high silver biosorption ability, and the potential application of bioreduction of [Ag(NH3)2]+ in nanoparticle preparation technology.
AB - The biomass of Aeromonas SH10 was proven to strongly absorb Ag+ and [Ag(NH3)2]+. The maximum uptake of [Ag(NH3)2]+ was 0.23 g(Ag) g-1(cell dry weight), higher than that of Ag+. Fourier transform infrared spectroscopy spectra analysis indicated that some organic groups, such as amide and ionized carboxyl in the cell wall, played an important role in the process of biosorption. After SH10 cells were suspended in the aqueous solution of [Ag(NH3)2]+ under 60°C for more than 12 h, [Ag(NH3)2]+ was reduced to Ag(0), which was demonstrated by the characteristic absorbance peak of elemental silver nanoparticle in UV-VIS spectrum. Scanning electron microscopy and transmission electron microscopy observation showed that nanoparticles were formed on the cell wall after reduction. These particles were then confirmed to be elemental silver crystal by energy dispersive X-ray spectroscopy, X-ray diffraction, and UV-VIS analysis. This study demonstrated the potential use of Aeromonas SH10 in silver-containing wastewater treatment due to its high silver biosorption ability, and the potential application of bioreduction of [Ag(NH3)2]+ in nanoparticle preparation technology.
KW - Bioreduction
KW - Biosorption
KW - Diamine silver complex
KW - Nanoparticle
KW - Silver ion
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U2 - 10.1007/s12010-007-8006-1
DO - 10.1007/s12010-007-8006-1
M3 - Article
C2 - 18025596
SN - 0273-2289
VL - 143
SP - 54
EP - 62
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
IS - 1
ER -