Accurate identification of non-polyadenylated RNA using a custom human cytomegalovirus tiling array

Ying Huang, Charles Warden, Hua Zhu

Research output: Contribution to journalArticle

Abstract

Most eukaryotes regulate commonly translation by polyadenylation of messenger RNA. RNA sequences without 3' polyadenylation are usually quickly degraded, thus finely tuning the expression of gene transcripts. Some viruses are known to regulate mRNA levels via polyadenylation. A method is described that utilizes a customized tiling array to determine if any human cytomegalovirus (HCMV) transcripts are subject to regulation via de-polyadenylation and subsequent mRNA decay. The results of this study indicate that the highly expressed intron of large non-coding RNA (lncRNA5) that is not polyadenylated, as documented previously. This differential polyadenylation protocol can also be modified for other tiling array applications (such as ChIP-chip analysis).

Original languageEnglish (US)
Pages (from-to)90-93
Number of pages4
JournalJournal of Virological Methods
Volume187
Issue number1
DOIs
StatePublished - Jan 1 2013

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Polyadenylation
Cytomegalovirus
RNA
Untranslated RNA
Messenger RNA
RNA Stability
Eukaryota
Introns
Viruses
Gene Expression

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

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Accurate identification of non-polyadenylated RNA using a custom human cytomegalovirus tiling array. / Huang, Ying; Warden, Charles; Zhu, Hua.

In: Journal of Virological Methods, Vol. 187, No. 1, 01.01.2013, p. 90-93.

Research output: Contribution to journalArticle

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