Activation of locus coeruleus by prefrontal cortex is mediated by excitatory amino acid inputs

Eiichi Jodo, Gary Aston Jones

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

We examined the role of excitatory amino acids (EAAs) in activation of noradrenergic locus coeruleus (LC) neurons evoked by electrical stimulation of the medial prefrontal cortex (mPFC) in halothane-anesthetized rats. Microinfusion of the specific N-methyl-Daspartate antagonist 2-amino-5- phosphonopentanoic acid (AP5, 50 or 100 μM) into the LC significantly suppressed LC responses evoked by mPFC stimulation. Microinfusion of the selective non-NMDA antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 25 or 50 μM) also significantly reduced evoked LC responses. Simultaneous microinfusion of both AP5 and CNQX considerably increased the proportion of LC neurons which exhibited complete suppression of evoked responses (81%), compared to either AP5 or CNQX alone (~ 50% each). These results indicate that LC activation by mPFC stimulation is mediated by both NMDA- and non- NMDA-type EAA channels.

Original languageEnglish (US)
Pages (from-to)327-332
Number of pages6
JournalBrain research
Volume768
Issue number1-2
DOIs
StatePublished - Sep 12 1997

Fingerprint

Excitatory Amino Acids
Locus Coeruleus
Prefrontal Cortex
6-Cyano-7-nitroquinoxaline-2,3-dione
N-Methylaspartate
Minor Lymphocyte Stimulatory Loci
2-Amino-5-phosphonovalerate
Neurons
Halothane
Electric Stimulation

All Science Journal Classification (ASJC) codes

  • Clinical Neurology
  • Molecular Biology
  • Neuroscience(all)
  • Developmental Biology

Keywords

  • 2-Amino-5-phosphonopentanoic acid
  • 6-Cyano-7-nitroquinoxaline-2,3-dione
  • Excitatory amino acid
  • Locus coeruleus
  • Norepinephrine
  • Prefrontal cortex

Cite this

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abstract = "We examined the role of excitatory amino acids (EAAs) in activation of noradrenergic locus coeruleus (LC) neurons evoked by electrical stimulation of the medial prefrontal cortex (mPFC) in halothane-anesthetized rats. Microinfusion of the specific N-methyl-Daspartate antagonist 2-amino-5- phosphonopentanoic acid (AP5, 50 or 100 μM) into the LC significantly suppressed LC responses evoked by mPFC stimulation. Microinfusion of the selective non-NMDA antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 25 or 50 μM) also significantly reduced evoked LC responses. Simultaneous microinfusion of both AP5 and CNQX considerably increased the proportion of LC neurons which exhibited complete suppression of evoked responses (81{\%}), compared to either AP5 or CNQX alone (~ 50{\%} each). These results indicate that LC activation by mPFC stimulation is mediated by both NMDA- and non- NMDA-type EAA channels.",
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Activation of locus coeruleus by prefrontal cortex is mediated by excitatory amino acid inputs. / Jodo, Eiichi; Aston Jones, Gary.

In: Brain research, Vol. 768, No. 1-2, 12.09.1997, p. 327-332.

Research output: Contribution to journalArticle

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