Abstract
We examined the role of excitatory amino acids (EAAs) in activation of noradrenergic locus coeruleus (LC) neurons evoked by electrical stimulation of the medial prefrontal cortex (mPFC) in halothane-anesthetized rats. Microinfusion of the specific N-methyl-Daspartate antagonist 2-amino-5- phosphonopentanoic acid (AP5, 50 or 100 μM) into the LC significantly suppressed LC responses evoked by mPFC stimulation. Microinfusion of the selective non-NMDA antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 25 or 50 μM) also significantly reduced evoked LC responses. Simultaneous microinfusion of both AP5 and CNQX considerably increased the proportion of LC neurons which exhibited complete suppression of evoked responses (81%), compared to either AP5 or CNQX alone (~ 50% each). These results indicate that LC activation by mPFC stimulation is mediated by both NMDA- and non- NMDA-type EAA channels.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 327-332 |
| Number of pages | 6 |
| Journal | Brain research |
| Volume | 768 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Sep 12 1997 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Clinical Neurology
- Molecular Biology
- Neuroscience(all)
- Developmental Biology
Keywords
- 2-Amino-5-phosphonopentanoic acid
- 6-Cyano-7-nitroquinoxaline-2,3-dione
- Excitatory amino acid
- Locus coeruleus
- Norepinephrine
- Prefrontal cortex
Cite this
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Activation of locus coeruleus by prefrontal cortex is mediated by excitatory amino acid inputs. / Jodo, Eiichi; Aston-Jones, Gary.
In: Brain research, Vol. 768, No. 1-2, 12.09.1997, p. 327-332.Research output: Contribution to journal › Article
TY - JOUR
T1 - Activation of locus coeruleus by prefrontal cortex is mediated by excitatory amino acid inputs
AU - Jodo, Eiichi
AU - Aston-Jones, Gary
PY - 1997/9/12
Y1 - 1997/9/12
N2 - We examined the role of excitatory amino acids (EAAs) in activation of noradrenergic locus coeruleus (LC) neurons evoked by electrical stimulation of the medial prefrontal cortex (mPFC) in halothane-anesthetized rats. Microinfusion of the specific N-methyl-Daspartate antagonist 2-amino-5- phosphonopentanoic acid (AP5, 50 or 100 μM) into the LC significantly suppressed LC responses evoked by mPFC stimulation. Microinfusion of the selective non-NMDA antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 25 or 50 μM) also significantly reduced evoked LC responses. Simultaneous microinfusion of both AP5 and CNQX considerably increased the proportion of LC neurons which exhibited complete suppression of evoked responses (81%), compared to either AP5 or CNQX alone (~ 50% each). These results indicate that LC activation by mPFC stimulation is mediated by both NMDA- and non- NMDA-type EAA channels.
AB - We examined the role of excitatory amino acids (EAAs) in activation of noradrenergic locus coeruleus (LC) neurons evoked by electrical stimulation of the medial prefrontal cortex (mPFC) in halothane-anesthetized rats. Microinfusion of the specific N-methyl-Daspartate antagonist 2-amino-5- phosphonopentanoic acid (AP5, 50 or 100 μM) into the LC significantly suppressed LC responses evoked by mPFC stimulation. Microinfusion of the selective non-NMDA antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 25 or 50 μM) also significantly reduced evoked LC responses. Simultaneous microinfusion of both AP5 and CNQX considerably increased the proportion of LC neurons which exhibited complete suppression of evoked responses (81%), compared to either AP5 or CNQX alone (~ 50% each). These results indicate that LC activation by mPFC stimulation is mediated by both NMDA- and non- NMDA-type EAA channels.
KW - 2-Amino-5-phosphonopentanoic acid
KW - 6-Cyano-7-nitroquinoxaline-2,3-dione
KW - Excitatory amino acid
KW - Locus coeruleus
KW - Norepinephrine
KW - Prefrontal cortex
UR - http://www.scopus.com/inward/record.url?scp=0030798727&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030798727&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/S0006-8993(97)00703-8
DO - https://doi.org/10.1016/S0006-8993(97)00703-8
M3 - Article
C2 - 9369332
VL - 768
SP - 327
EP - 332
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1-2
ER -