Acute osmotic tolerance of cultured cells of the oyster pathogen Perkinsus marinus (Apicomplexa: Perkinsida)

Eugene M. Burreson, Lisa M. Ragone Calvo, Jerome F. La Peyre, Fay Counts, Kennedy T. Paynter

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


Cultured Perkinsus marinus cells were exposed for 24 hr to salinities of 0, 3, 6, 9, 12 and 22 ppt at temperatures of 1, 5, 10, 15 and 28°C in artificial seawater (ASW) and to the same salinities at 28°C in ASW with the osmotic concentration adjusted with sucrose to the equivalent of 22 ppt. At 28°C mortality increased as salinity decreased below 22 ppt. Mortality was greater than 99% at 0 ppt and greater than 90% at 3 ppt. Mortality was 70% at 6 ppt, 43% at 9 ppt and 20% at 12 ppt. Mortality was low (<5%) and equal to that at 22 ppt in all treatments where osmotic concentration was maintained with sucrose. Mortality occurred rapidly, within 5 min of exposure to experimental conditions. In the region where mortality was most sensitive to salinity changes (6-12 ppt), lower temperature caused an increase in mortality, but the temperature effect was significant only at 9 ppt.

Original languageEnglish (US)
Pages (from-to)575-582
Number of pages8
JournalComparative Biochemistry and Physiology -- Part A: Physiology
Issue number3
StatePublished - Nov 1994
Externally publishedYes

ASJC Scopus subject areas

  • Physiology


  • Cultured cells
  • Osmotic tolerance
  • Oyster pathogen
  • Perkinsus marinus


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