TY - JOUR
T1 - Acute osmotic tolerance of cultured cells of the oyster pathogen Perkinsus marinus (Apicomplexa
T2 - Perkinsida)
AU - Burreson, Eugene M.
AU - Ragone Calvo, Lisa M.
AU - La Peyre, Jerome F.
AU - Counts, Fay
AU - Paynter, Kennedy T.
PY - 1994/11
Y1 - 1994/11
N2 - Cultured Perkinsus marinus cells were exposed for 24 hr to salinities of 0, 3, 6, 9, 12 and 22 ppt at temperatures of 1, 5, 10, 15 and 28°C in artificial seawater (ASW) and to the same salinities at 28°C in ASW with the osmotic concentration adjusted with sucrose to the equivalent of 22 ppt. At 28°C mortality increased as salinity decreased below 22 ppt. Mortality was greater than 99% at 0 ppt and greater than 90% at 3 ppt. Mortality was 70% at 6 ppt, 43% at 9 ppt and 20% at 12 ppt. Mortality was low (<5%) and equal to that at 22 ppt in all treatments where osmotic concentration was maintained with sucrose. Mortality occurred rapidly, within 5 min of exposure to experimental conditions. In the region where mortality was most sensitive to salinity changes (6-12 ppt), lower temperature caused an increase in mortality, but the temperature effect was significant only at 9 ppt.
AB - Cultured Perkinsus marinus cells were exposed for 24 hr to salinities of 0, 3, 6, 9, 12 and 22 ppt at temperatures of 1, 5, 10, 15 and 28°C in artificial seawater (ASW) and to the same salinities at 28°C in ASW with the osmotic concentration adjusted with sucrose to the equivalent of 22 ppt. At 28°C mortality increased as salinity decreased below 22 ppt. Mortality was greater than 99% at 0 ppt and greater than 90% at 3 ppt. Mortality was 70% at 6 ppt, 43% at 9 ppt and 20% at 12 ppt. Mortality was low (<5%) and equal to that at 22 ppt in all treatments where osmotic concentration was maintained with sucrose. Mortality occurred rapidly, within 5 min of exposure to experimental conditions. In the region where mortality was most sensitive to salinity changes (6-12 ppt), lower temperature caused an increase in mortality, but the temperature effect was significant only at 9 ppt.
KW - Cultured cells
KW - Osmotic tolerance
KW - Oyster pathogen
KW - Perkinsus marinus
UR - http://www.scopus.com/inward/record.url?scp=0028104642&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028104642&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/0300-9629(94)90196-1
DO - https://doi.org/10.1016/0300-9629(94)90196-1
M3 - Article
C2 - 8529003
SN - 0300-9629
VL - 109
SP - 575
EP - 582
JO - Comparative Biochemistry and Physiology -- Part A: Physiology
JF - Comparative Biochemistry and Physiology -- Part A: Physiology
IS - 3
ER -