Acute Stress Persistently Alters Locus Coeruleus Function and Anxiety-like Behavior in Adolescent Rats

Olga Borodovitsyna, Matthew D. Flamini, Daniel Chandler

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Stress is a physiological state characterized by altered neuroendocrine signaling, behavioral arousal, and anxiety. Chronic or traumatic stress may predispose individuals for multiple somatic and psychiatric illnesses. The locus coeruleus (LC) is a major node in the stress response that integrates input from multiple stress responsive neural circuits and releases norepinephrine (NE) throughout the central nervous system (CNS) to promote vigilance and anxiety. Many mood disorders associated with prior stress are characterized by chronically altered noradrenergic signaling, yet the long-term impact of an acute stressor on LC function is not clear. To determine how acute stress could affect anxiety-like behavior as well as LC function at immediate and extended time points, rats underwent simultaneous exposure to physical restraint and predator odor. Rats underwent behavioral testing immediately or one week after stressor exposure and were then sacrificed for whole-cell patch-clamp recordings of LC neurons. Stress caused an immediate increase in anxiety-like behaviors in the elevated plus maze (EPM), as well decreased excitatory synaptic transmission and increased spontaneous discharge in LC neurons. These effects persisted for seven days after stress. Importantly, the excitability of LC neurons was increased one week post-stress, but not immediately after, suggesting a long-term adaptation by the system. Rats tested in the open field one week after stress also showed increased anxiety-like behaviors. These findings show that a single acute stressor is capable of precipitating long-lasting changes in the LC function that may be related to some of the behavioral effects of stress, potentially contributing to stress-induced disease pathogenesis.

Original languageEnglish (US)
Pages (from-to)7-19
Number of pages13
JournalNeuroscience
Volume373
DOIs
StatePublished - Mar 1 2018

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Adolescent Behavior
Locus Coeruleus
Anxiety
Neurons
Physical Restraint
Arousal
Mood Disorders
Synaptic Transmission
Psychiatry
Norepinephrine
Central Nervous System

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

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abstract = "Stress is a physiological state characterized by altered neuroendocrine signaling, behavioral arousal, and anxiety. Chronic or traumatic stress may predispose individuals for multiple somatic and psychiatric illnesses. The locus coeruleus (LC) is a major node in the stress response that integrates input from multiple stress responsive neural circuits and releases norepinephrine (NE) throughout the central nervous system (CNS) to promote vigilance and anxiety. Many mood disorders associated with prior stress are characterized by chronically altered noradrenergic signaling, yet the long-term impact of an acute stressor on LC function is not clear. To determine how acute stress could affect anxiety-like behavior as well as LC function at immediate and extended time points, rats underwent simultaneous exposure to physical restraint and predator odor. Rats underwent behavioral testing immediately or one week after stressor exposure and were then sacrificed for whole-cell patch-clamp recordings of LC neurons. Stress caused an immediate increase in anxiety-like behaviors in the elevated plus maze (EPM), as well decreased excitatory synaptic transmission and increased spontaneous discharge in LC neurons. These effects persisted for seven days after stress. Importantly, the excitability of LC neurons was increased one week post-stress, but not immediately after, suggesting a long-term adaptation by the system. Rats tested in the open field one week after stress also showed increased anxiety-like behaviors. These findings show that a single acute stressor is capable of precipitating long-lasting changes in the LC function that may be related to some of the behavioral effects of stress, potentially contributing to stress-induced disease pathogenesis.",
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Acute Stress Persistently Alters Locus Coeruleus Function and Anxiety-like Behavior in Adolescent Rats. / Borodovitsyna, Olga; Flamini, Matthew D.; Chandler, Daniel.

In: Neuroscience, Vol. 373, 01.03.2018, p. 7-19.

Research output: Contribution to journalArticle

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