Adenosine A2a receptor-mediated inhibition of rod opsin mRNA expression in tiger salamander

Peter D. Alfinito, Roshni Alli, Ellen Townes-Anderson

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The neuromodulator adenosine mediates dark-adaptive changes in retinal photoreceptors through A2a receptors. In cold-blooded vertebrates, opsin mRNA expression is lower at night than during the day. In the present study, we tested whether adenosine could inhibit opsin mRNA expression in cultured rod cells and if endogenous adenosine acts to suppress opsin mRNA in the intact retina at night. Semi-quantitative in situ hybridization showed that treatment with 100 μm of the A2a/A2b agonist N6-[2-(3,5-dimethoxyphenyl)-2-(2-methylphenyl)-ethyl]adenosine (DPMA) reduced opsin mRNA 41% in cultured rod cells. The effect of DPMA was blocked by 10 μm of the A2a antagonist 8-(3-chlorostyryl)caffeine (CSC) but not by 10 μm of the A2b antagonist alloxazine. One micromolar adenosine alone had no effect on opsin mRNA. However, in the presence of the adenosine deaminase inhibitor erythro-9-(2-hydroxy-3-nonyl)adenine hydrochloride (EHNA), 1 μm adenosine reduced opsin mRNA 61%. EHNA alone reduced opsin mRNA by 26%. Consistent with an A2a receptor mechanism, 100 μm forskolin (adenylate cyclase agonist) decreased opsin mRNA 34%. Finally, northern blots showed that intravitreal injection of 10 μm CSC at night increased opsin I mRNA 38%. Thus, endogenous adenosine suppresses rod opsin I mRNA expression at night; in vitro results indicate this reduction occurs through A2a-like receptor binding and stimulation of adenylate cyclase activity.

Original languageEnglish (US)
Pages (from-to)665-672
Number of pages8
JournalJournal of neurochemistry
Volume83
Issue number3
DOIs
StatePublished - Nov 2002

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Keywords

  • A receptor
  • Adenosine
  • MRNA expression
  • Opsin
  • Retina
  • Rod photoreceptor

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