Alternative cleavage and polyadenylation: The long and short of it

Bin Tian; James L. Manley

doi:https://doi.org/10.1016/j.tibs.2013.03.005

Alternative cleavage and polyadenylation: The long and short of it

Bin Tian, James L. Manley

Rutgers, The State University

Research output: Contribution to journal › Review article › peer-review

210 Scopus citations

Abstract

Cleavage and polyadenylation (C/P) of nascent transcripts is essential for maturation of the 3' ends of most eukaryotic mRNAs. Over the past three decades, biochemical studies have elucidated the machinery responsible for the seemingly simple C/P reaction. Recent genomic analyses have indicated that most eukaryotic genes have multiple cleavage and polyadenylation sites (pAs), leading to transcript isoforms with different coding potentials and/or variable 3' untranslated regions (UTRs). As such, alternative cleavage and polyadenylation (APA) is an important layer of gene regulation impacting mRNA metabolism. Here, we review our current understanding of APA and recent progress in this field.

Original language	English (US)
Pages (from-to)	312-320
Number of pages	9
Journal	Trends in Biochemical Sciences
Volume	38
Issue number	6
DOIs	https://doi.org/10.1016/j.tibs.2013.03.005
State	Published - Jun 2013

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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https://doi.org/10.1016/j.tibs.2013.03.005

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title = "Alternative cleavage and polyadenylation: The long and short of it",

abstract = "Cleavage and polyadenylation (C/P) of nascent transcripts is essential for maturation of the 3' ends of most eukaryotic mRNAs. Over the past three decades, biochemical studies have elucidated the machinery responsible for the seemingly simple C/P reaction. Recent genomic analyses have indicated that most eukaryotic genes have multiple cleavage and polyadenylation sites (pAs), leading to transcript isoforms with different coding potentials and/or variable 3' untranslated regions (UTRs). As such, alternative cleavage and polyadenylation (APA) is an important layer of gene regulation impacting mRNA metabolism. Here, we review our current understanding of APA and recent progress in this field.",

author = "Bin Tian and Manley, {James L.}",

note = "Funding Information: We thank our laboratory members for helpful discussions. We apologize for not citing many important papers related to the topic owing to space limitation. This work was funded by grants GM 84089 (B.T.) and GM 28983 (J.L.M.) from National Institutes of Health.",

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T1 - Alternative cleavage and polyadenylation

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AU - Tian, Bin

AU - Manley, James L.

N1 - Funding Information: We thank our laboratory members for helpful discussions. We apologize for not citing many important papers related to the topic owing to space limitation. This work was funded by grants GM 84089 (B.T.) and GM 28983 (J.L.M.) from National Institutes of Health.

PY - 2013/6

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N2 - Cleavage and polyadenylation (C/P) of nascent transcripts is essential for maturation of the 3' ends of most eukaryotic mRNAs. Over the past three decades, biochemical studies have elucidated the machinery responsible for the seemingly simple C/P reaction. Recent genomic analyses have indicated that most eukaryotic genes have multiple cleavage and polyadenylation sites (pAs), leading to transcript isoforms with different coding potentials and/or variable 3' untranslated regions (UTRs). As such, alternative cleavage and polyadenylation (APA) is an important layer of gene regulation impacting mRNA metabolism. Here, we review our current understanding of APA and recent progress in this field.

AB - Cleavage and polyadenylation (C/P) of nascent transcripts is essential for maturation of the 3' ends of most eukaryotic mRNAs. Over the past three decades, biochemical studies have elucidated the machinery responsible for the seemingly simple C/P reaction. Recent genomic analyses have indicated that most eukaryotic genes have multiple cleavage and polyadenylation sites (pAs), leading to transcript isoforms with different coding potentials and/or variable 3' untranslated regions (UTRs). As such, alternative cleavage and polyadenylation (APA) is an important layer of gene regulation impacting mRNA metabolism. Here, we review our current understanding of APA and recent progress in this field.

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Alternative cleavage and polyadenylation: The long and short of it

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