Alternative cleavage and polyadenylation: The long and short of it

Bin Tian, James L. Manley

Research output: Contribution to journalReview articlepeer-review

210 Scopus citations


Cleavage and polyadenylation (C/P) of nascent transcripts is essential for maturation of the 3' ends of most eukaryotic mRNAs. Over the past three decades, biochemical studies have elucidated the machinery responsible for the seemingly simple C/P reaction. Recent genomic analyses have indicated that most eukaryotic genes have multiple cleavage and polyadenylation sites (pAs), leading to transcript isoforms with different coding potentials and/or variable 3' untranslated regions (UTRs). As such, alternative cleavage and polyadenylation (APA) is an important layer of gene regulation impacting mRNA metabolism. Here, we review our current understanding of APA and recent progress in this field.

Original languageEnglish (US)
Pages (from-to)312-320
Number of pages9
JournalTrends in Biochemical Sciences
Issue number6
StatePublished - Jun 2013

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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