Ammoniation to reduce the toxicity of endophyte-infected tall fescue seed fed to rats.

A. Simeone, M. L. Westendorf, R. E. Tucker, L. P. Bush, G. E. Mitchell

Research output: Contribution to journalArticlepeer-review


To assess the efficacy of ammoniation in the detoxification of endophyte-infected tall fescue (Festuca arundinacea Schreb), 40 male Harlan Sprague-Dawley rats were randomly assigned to the following four treatments for 28 d: endophyte-free (E-), endophyte-infected (E+), ammoniated (2% dry matter basis, 7 d) endophyte-free (AE-), and ammoniated endophyte-infected (AE+) tall fescue seed. Total pyrrolizidine alkaloid (N-acetyl and N-formyl loline) and ergovaline contents of endophyte-infected fescue seed were reduced 24 and 54%, respectively, by ammoniation. Endophyte-infected treatment groups had lower (P < 0.01) daily feed intakes (DFI), daily weight gains (DWG), feed efficiencies, and primary serum hemagglutination titers to sheep red blood cell (SRBC) immunization than endophyte-free treatment groups. Performance parameters were higher (P < 0.01) for ammoniated diets in comparison to non-ammoniated diets; however, anti-SRBC titers were not significantly different. When compared to the E+ diet, the AE+ diet increased (P < 0.01) DFI (24%), DWG (41%) and feed efficiency (13%).

Original languageEnglish (US)
Pages (from-to)373-385
Number of pages13
JournalDrug and chemical toxicology
Issue number3
StatePublished - Aug 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Chemical Health and Safety
  • Pharmacology


Dive into the research topics of 'Ammoniation to reduce the toxicity of endophyte-infected tall fescue seed fed to rats.'. Together they form a unique fingerprint.

Cite this