An improved method for the determination of green and black tea polyphenols in biomatrices by high-performance liquid chromatography with coulometric array detection

Mao Jung Lee, Saileta Prabhu, Xiaofeng Meng, Chuan Li, Chung S. Yang

Research output: Contribution to journalArticlepeer-review

147 Scopus citations

Abstract

Tea polyphenols are strong antioxidants and are believed to have beneficial health effects. However, the blood and tissue levels of these compounds are not well characterized because of a lack of suitable analytical methods for the biological resolution of these compounds. Previously, we developed methods for the analysis of three green tea catechins. Now we report an improved method for the measurement of the levels of the different catechins and theaflavins in biological fluids and tissues. The method includes digestion of the plasma, urine, or tissue samples with β-D- glucuronidase and sulfatase, followed by extraction with ethyl acetate and subsequent separation by reversed-phase high-performance liquid chromatography (HPLC). The polyphenols are identified on the basis of their retention times, spectral analysis, and electrochemical behavior across an array of electrodes. In a single HPLC run, it is possible to determine the major catechins and theaflavins as well as some of the catechin metabolites. The detection limits for catechins and theaflavins are from 5 to 10 ng/ml of saliva, plasma, or urine. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)164-169
Number of pages6
JournalAnalytical Biochemistry
Volume279
Issue number2
DOIs
StatePublished - Mar 15 2000

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biophysics
  • Biochemistry
  • Cell Biology

Keywords

  • Black tea
  • Catechins
  • Coulochem detection
  • Green tea
  • HPLC
  • Plasma
  • Polyphenols
  • Saliva
  • Theaflavins

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