Analysis of gene cassettes with integrons from biofilm samples in coking wastewater and phenol-degrading bacteria

Liye Zhang, Chenguang Zhu, Xiaojun Zhang, Liping Zhao

Research output: Contribution to journalArticlepeer-review

Abstract

To investigate the structure of integron-gene cassette system of the microorganisms in coking wastewater and analyze the gene cassette sequences of two strains of phenol degrading bacteria isolated from identical activated sludge, the gene cassette PCR was performed using the primers designed according to the conserved sequence of the 59 base element structure and the integrase encoding sequence from class 1 integrons. The result showed that all the biofilm samples in the coking wastewater and isolated bacteria produced positive bands. The clones derived from the gene cassette clone libraries of two bacterial strains Alcaligenes faecalis IS-17 and IS-46 were sequenced and further analyzed by sequence alignment and prediction of their functions. The structures of the gene cassettes were compared with the ones previously reported, in the boundary sites for primer binding, length of region between the ORF and 59be conserved sequence. It was concluded that the gene cassettes structure is widespread in the microbial communities of the coking wastewater biofilm. This work confirms that the gene cassette PCR is an effective method to reveal novel genes from the environment or from the microorganisms.

Original languageEnglish (US)
Pages (from-to)644-649
Number of pages6
JournalChinese Journal of Applied and Environmental Biology
Volume14
Issue number5
DOIs
StatePublished - Oct 25 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Pollution
  • Genetics
  • Agronomy and Crop Science

Keywords

  • Coking wastewater
  • Gene cassette
  • Gene cassette PCR
  • Integron

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