Analysis of microRNA effector functions in vitro

Bingbing Wang, John G. Doench, Carl D. Novina

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Analyses of gene functions in vitro provide the means to dissect biological phenomena. Development of cell-free assays for transcription, splicing, and translation has yielded great insights into macromolecular interactions and functions required for these processes. This article discusses development of cell-free assays to test macromolecular interactions and activities required for microRNA effector functions. This chapter also compares in vitro analyses to complementary studies in cells and the technical considerations that permit molecular analysis of microRNA function in cell-free conditions.

Original languageEnglish (US)
Pages (from-to)91-104
Number of pages14
JournalMethods
Volume43
Issue number2
DOIs
StatePublished - Oct 1 2007

Fingerprint

MicroRNAs
Assays
Biological Phenomena
Transcription
Genes
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Molecular Biology

Keywords

  • Cell-free
  • Gene silencing
  • MicroRNA
  • RNA interference
  • Translation
  • siRNA

Cite this

Wang, Bingbing ; Doench, John G. ; Novina, Carl D. / Analysis of microRNA effector functions in vitro. In: Methods. 2007 ; Vol. 43, No. 2. pp. 91-104.
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Analysis of microRNA effector functions in vitro. / Wang, Bingbing; Doench, John G.; Novina, Carl D.

In: Methods, Vol. 43, No. 2, 01.10.2007, p. 91-104.

Research output: Contribution to journalArticle

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