Analysis of the meiotic role of the mitochondrial ribosomal proteins Mrps17 and Mrp137 in Saccharomyces cerevisiae

Sean H. Hanlon, Zhiheng Xu, David N. Norris, Andrew K. Vershon

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Sporulation in the yeast Saccharomyces cerevisiae is a complex and tightly regulated pathway that involves the induction of a large number of genes. We have identified MRPS17 in a cDNA library enriched for sporulation-specific genes. Homology searches show that the first one-third of Mrps17 has strong sequence similarity to bacterial S17 proteins, suggesting that Mrps17 is a potential mitochondrial ribosomal protein. This is further supported by the fact that mrps17Δ cells are respiratory-deficient and that a Mrps17-GFP fusion localizes to the mitochondria. We have confirmed by Northern blot analysis that both MRPS17 and MRPL37 are strongly induced during the middle stages of sporulation and that this induction is dependent on the presence of a middle sporulation element (MSE) in the promoters of these genes. Interestingly, we found that Mrps17 and Mrpl37, but not other mitochondrial ribosomal proteins, accumulate during the middle stages of sporulation. These results suggest that Mrps17 and Mrpl37 may have additional meiosis-specific roles.

Original languageEnglish (US)
Pages (from-to)1241-1252
Number of pages12
JournalYeast
Volume21
Issue number15
DOIs
StatePublished - Nov 2004

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Bioengineering
  • Genetics
  • Biochemistry
  • Biotechnology

Keywords

  • Meiosis
  • Ribosome and middle sporulation element (MSE)
  • Sporulation

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