Anthocyanins in blackcurrant effectively prevent the formation of advanced glycation end products by trapping methylglyoxal

TY - JOUR

T1 - Anthocyanins in blackcurrant effectively prevent the formation of advanced glycation end products by trapping methylglyoxal

AU - Chen, Xiu Yu

AU - Huang, I. Min

AU - Hwang, Lucy Sun

AU - Ho, Chi Tang

AU - Li, Shiming

AU - Lo, Chih Yu

N1 - Funding Information: This work was supported by the National Science Council under the grant numbers, NSC 97-2313-B-415-002-MY2 .

PY - 2014

Y1 - 2014

N2 - Carbonyl stress is the major consequence for advanced glycation end products (AGEs). Polyphenols have been shown to inhibit the formation of AGEs. However, little is known about whether anthocyanins contribute to trapping methylglyoxal (MGO) and reducing carbonyl stress. Blackcurrant (Ribes nigrum) extract (BE) is known to have high content of anthocyanins. When an aliquot of 7.5 mg/mL BE sample was incubated with 2.9 mM MGO in pH 7.4 phosphate buffer solution (PBS) for an hour, 36.75% MGO decrease was observed. The BE extract sample was separated and purified by Sephadex LH-20 and reverse phase C18 column chromatography. Delphinidin-3-rutinoside (D3R) and cyanidin-3-rutinoside (C3R) were identified as major pigments in BE sample. Some 71.45 and 78.72% of D3R and C3R, respectively, remained when they were incubated alone in a phosphate buffer solution at 37 °C for 1 h, but only 2.48 and 1.83% of D3R and C3R, respectively, remained when individually incubated with MGO. D3R- and C3R-mono-MGO adducts were characterized by HPLC-MSn.

AB - Carbonyl stress is the major consequence for advanced glycation end products (AGEs). Polyphenols have been shown to inhibit the formation of AGEs. However, little is known about whether anthocyanins contribute to trapping methylglyoxal (MGO) and reducing carbonyl stress. Blackcurrant (Ribes nigrum) extract (BE) is known to have high content of anthocyanins. When an aliquot of 7.5 mg/mL BE sample was incubated with 2.9 mM MGO in pH 7.4 phosphate buffer solution (PBS) for an hour, 36.75% MGO decrease was observed. The BE extract sample was separated and purified by Sephadex LH-20 and reverse phase C18 column chromatography. Delphinidin-3-rutinoside (D3R) and cyanidin-3-rutinoside (C3R) were identified as major pigments in BE sample. Some 71.45 and 78.72% of D3R and C3R, respectively, remained when they were incubated alone in a phosphate buffer solution at 37 °C for 1 h, but only 2.48 and 1.83% of D3R and C3R, respectively, remained when individually incubated with MGO. D3R- and C3R-mono-MGO adducts were characterized by HPLC-MSn.

KW - Anthocyanins

KW - Blackcurrant

KW - Dicarbonyls

KW - Methylglyoxal-adducts

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U2 - https://doi.org/10.1016/j.jff.2014.03.025

DO - https://doi.org/10.1016/j.jff.2014.03.025

M3 - Article

SN - 1756-4646

VL - 8

SP - 259

EP - 268

JO - Journal of Functional Foods

JF - Journal of Functional Foods

IS - 1

ER -