Antibodies to N-homocysteinylated albumin as a marker for early-onset coronary artery disease in men

Anetta Undas, Milosz Jankowski, Magdalena Twardowska, Agnieszka Padjas, Hieronim Jakubowski, Andrzej Szczeklik

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

N-homocysteinylated (NE-Hcy) proteins and corresponding antibodies have recently been discovered in humans and animals. Increased autoimmune response to NE-Hcy-proteins has been reported in stroke patients.The aim of the present study was to investigate whether antibodies against N-homocysteinylated albumin are associated with coronary artery disease (CAD). We studied 88 male patients aged 50 years or under with angiographically documented CAD and 100 age-matched apparently healthy men as controls. Serum levels of IgG antibodies against NE-Hcy-albumin were determined using an enzyme-linked immunosorbent assay. Seropositivity to anti-NE-Hcy-albumin antibodies was 5-fold more frequent in CAD patients than in controls (52.3% vs 10.0%; p<0.0001). Plasma Hcy levels in CAD patients were also significantly higher in the former than in the latter group (medians, 13.0 μM vs 12.1 μM; p=0.026). Importantly, 41.2% of subjects with plasma total Hcy > 14.5 mM were seropositive compared with 25.5% of normohomocysteinemic individuals (p=0.048).There was a weak correlation between anti-NE-Hcy-albumin antibodies and Hcy levels (r=0.16; p=0.03). By multivariate logistic regression analysis, seropositivity to anti-NE-Hcy-albumin antibodies was an independent predictor of early CAD (OR, 14.82; 95% CI, 4.47 to 49.19; p=0.00002). Interestingly, anti-NE-Hcy-albumin antibodies were associated with C-reactive protein levels (r=0.24; p=0.002). Seropositivity to anti-NE-Hcy-albumin antibodies showed no association with the MTHFR C677T polymorphism. Our results suggest that seropositivity to antibodies against NE-homocysteinylated albumin is associated with early-onset CAD. An autoimmune response to NE-Hcy-albumin may represent a novel mechanism involved in the early development of CAD.

Original languageEnglish (US)
Pages (from-to)346-350
Number of pages5
JournalThrombosis and Haemostasis
Volume93
Issue number2
DOIs
StatePublished - Feb 2005

ASJC Scopus subject areas

  • Hematology

Keywords

  • Antibodies
  • Coronary artery disease
  • Homocysteine

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