Apoptosis is associated with modifications of bcl-2 mRNA AU-binding proteins

Martino Donnini; Andrea Lapucci; Laura Papucci; Ewa Witort; Alessio Tempestini; Gary Brewer; Anna Bevilacqua; Angelo Nicolin; Sergio Capaccioli; Nicola Schiavone

doi:https://doi.org/10.1006/bbrc.2001.5700

Apoptosis is associated with modifications of bcl-2 mRNA AU-binding proteins

Martino Donnini, Andrea Lapucci, Laura Papucci, Ewa Witort, Alessio Tempestini, Gary Brewer, Anna Bevilacqua, Angelo Nicolin, Sergio Capaccioli, Nicola Schiavone

Rutgers, The State University

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

The expression of genes requiring finely tuned control is regulated by a posttranscriptional mechanism involving mRNA A + U-rich elements (AREs) cooperating with ARE-binding proteins (AUBPs) in modulation of mRNA stability. We reported previously that an ARE in the bcl-2 mRNA 3′-untranslated region (3′-UTR) had destabilizing activity and was involved in bcl-2 downregulation during apoptosis in vitro. Here we demonstrate that the bcl-2 ARE complexes with a number of specific AUBPs, whose pattern undergoes changes following application of apoptotic stimuli. The caspase inhibitor Z-VAD-fmk strongly attenuates both bcl-2 mRNA decay and bcl-2 AUBP pattern changes elicited by apoptotic stimuli, indicating the involvement of bcl-2 AUBPs in bcl-2 mRNA stability control.

Original language	English (US)
Pages (from-to)	1063-1069
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	287
Issue number	5
DOIs	https://doi.org/10.1006/bbrc.2001.5700
State	Published - Oct 12 2001

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Keywords

AU-binding proteins
AU-rich elements
Apoptosis
Gene expression
Posttranscriptional control
bcl-2

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https://doi.org/10.1006/bbrc.2001.5700

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title = "Apoptosis is associated with modifications of bcl-2 mRNA AU-binding proteins",

abstract = "The expression of genes requiring finely tuned control is regulated by a posttranscriptional mechanism involving mRNA A + U-rich elements (AREs) cooperating with ARE-binding proteins (AUBPs) in modulation of mRNA stability. We reported previously that an ARE in the bcl-2 mRNA 3′-untranslated region (3′-UTR) had destabilizing activity and was involved in bcl-2 downregulation during apoptosis in vitro. Here we demonstrate that the bcl-2 ARE complexes with a number of specific AUBPs, whose pattern undergoes changes following application of apoptotic stimuli. The caspase inhibitor Z-VAD-fmk strongly attenuates both bcl-2 mRNA decay and bcl-2 AUBP pattern changes elicited by apoptotic stimuli, indicating the involvement of bcl-2 AUBPs in bcl-2 mRNA stability control.",

keywords = "AU-binding proteins, AU-rich elements, Apoptosis, Gene expression, Posttranscriptional control, bcl-2",

author = "Martino Donnini and Andrea Lapucci and Laura Papucci and Ewa Witort and Alessio Tempestini and Gary Brewer and Anna Bevilacqua and Angelo Nicolin and Sergio Capaccioli and Nicola Schiavone",

note = "Funding Information: This work was supported by grants from AIRC, MURST, and Ente Cassa di Risparmio di Firenze. A.L. is a recipient of a fellowship from FIRC.",

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doi = "https://doi.org/10.1006/bbrc.2001.5700",

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Apoptosis is associated with modifications of bcl-2 mRNA AU-binding proteins. / Donnini, Martino; Lapucci, Andrea; Papucci, Laura; Witort, Ewa; Tempestini, Alessio; Brewer, Gary; Bevilacqua, Anna; Nicolin, Angelo; Capaccioli, Sergio; Schiavone, Nicola.

In: Biochemical and Biophysical Research Communications, Vol. 287, No. 5, 12.10.2001, p. 1063-1069.

Research output: Contribution to journal › Article › peer-review

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AU - Lapucci, Andrea

AU - Papucci, Laura

AU - Witort, Ewa

AU - Tempestini, Alessio

AU - Brewer, Gary

AU - Bevilacqua, Anna

AU - Nicolin, Angelo

AU - Capaccioli, Sergio

AU - Schiavone, Nicola

N1 - Funding Information: This work was supported by grants from AIRC, MURST, and Ente Cassa di Risparmio di Firenze. A.L. is a recipient of a fellowship from FIRC.

PY - 2001/10/12

Y1 - 2001/10/12

N2 - The expression of genes requiring finely tuned control is regulated by a posttranscriptional mechanism involving mRNA A + U-rich elements (AREs) cooperating with ARE-binding proteins (AUBPs) in modulation of mRNA stability. We reported previously that an ARE in the bcl-2 mRNA 3′-untranslated region (3′-UTR) had destabilizing activity and was involved in bcl-2 downregulation during apoptosis in vitro. Here we demonstrate that the bcl-2 ARE complexes with a number of specific AUBPs, whose pattern undergoes changes following application of apoptotic stimuli. The caspase inhibitor Z-VAD-fmk strongly attenuates both bcl-2 mRNA decay and bcl-2 AUBP pattern changes elicited by apoptotic stimuli, indicating the involvement of bcl-2 AUBPs in bcl-2 mRNA stability control.

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KW - AU-binding proteins

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Apoptosis is associated with modifications of bcl-2 mRNA AU-binding proteins

Abstract

ASJC Scopus subject areas

Keywords

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