Apoptosis is associated with modifications of bcl-2 mRNA AU-binding proteins

Martino Donnini, Andrea Lapucci, Laura Papucci, Ewa Witort, Alessio Tempestini, Gary Brewer, Anna Bevilacqua, Angelo Nicolin, Sergio Capaccioli, Nicola Schiavone

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The expression of genes requiring finely tuned control is regulated by a posttranscriptional mechanism involving mRNA A + U-rich elements (AREs) cooperating with ARE-binding proteins (AUBPs) in modulation of mRNA stability. We reported previously that an ARE in the bcl-2 mRNA 3′-untranslated region (3′-UTR) had destabilizing activity and was involved in bcl-2 downregulation during apoptosis in vitro. Here we demonstrate that the bcl-2 ARE complexes with a number of specific AUBPs, whose pattern undergoes changes following application of apoptotic stimuli. The caspase inhibitor Z-VAD-fmk strongly attenuates both bcl-2 mRNA decay and bcl-2 AUBP pattern changes elicited by apoptotic stimuli, indicating the involvement of bcl-2 AUBPs in bcl-2 mRNA stability control.

Original languageEnglish (US)
Pages (from-to)1063-1069
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume287
Issue number5
DOIs
StatePublished - Oct 12 2001

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Keywords

  • AU-binding proteins
  • AU-rich elements
  • Apoptosis
  • Gene expression
  • Posttranscriptional control
  • bcl-2

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