Apoptotic-inducing epidioxysterols identified in hard clam (Meretrix lusoria)

TY - JOUR

T1 - Apoptotic-inducing epidioxysterols identified in hard clam (Meretrix lusoria)

AU - Pan, Min Hsiung

AU - Huang, Yu Ting

AU - Chang, Chi I.

AU - Ho, Chi Tang

AU - Pan, Bonnie Sun

N1 - Funding Information: This study was supported by the National Science Council NSC 93-2321-B-022-001, NSC 93-2313-B-022-004, and NSC 94-2321-B-022-001.

PY - 2007

Y1 - 2007

N2 - Hard clams (HC), Meretrix lusoria are a popular seafood and traditionally used as a Chinese remedy for liver disease and chronic hepatitis. The goal of this study was to identify compounds from hard clam, which are responsible for inducing apoptosis using bioassay-directed isolation. The effects of ethyl acetate extract of HC (HC-EA) on cell viability in human cancer cells were investigated. The ethyl acetate fraction was then subjected to separation and purification using silica gel column chromatography. The compounds showing strong apoptosis inducing activity were identified by spectral methods as epidioxysterols (EDS). The molecular mechanisms of epidioxysterolds-induced apoptosis as determined by annexin V apoptosis assay, DNA condensation, and sub-G1 DNA were investigated. The results suggest that induction of apoptosis by epidioxysterols may provide a pivotal mechanism for its cancer chemoprevention.

AB - Hard clams (HC), Meretrix lusoria are a popular seafood and traditionally used as a Chinese remedy for liver disease and chronic hepatitis. The goal of this study was to identify compounds from hard clam, which are responsible for inducing apoptosis using bioassay-directed isolation. The effects of ethyl acetate extract of HC (HC-EA) on cell viability in human cancer cells were investigated. The ethyl acetate fraction was then subjected to separation and purification using silica gel column chromatography. The compounds showing strong apoptosis inducing activity were identified by spectral methods as epidioxysterols (EDS). The molecular mechanisms of epidioxysterolds-induced apoptosis as determined by annexin V apoptosis assay, DNA condensation, and sub-G1 DNA were investigated. The results suggest that induction of apoptosis by epidioxysterols may provide a pivotal mechanism for its cancer chemoprevention.

KW - Apoptosis

KW - Epidioxysterols

KW - Hard clam

KW - Meretrix lusoria

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U2 - 10.1016/j.foodchem.2006.06.019

DO - 10.1016/j.foodchem.2006.06.019

M3 - Article

SN - 0308-8146

VL - 102

SP - 788

EP - 795

JO - Food Chemistry

JF - Food Chemistry

IS - 3

ER -