Arbitrarily primed PCR fingerprinting of RNA

TY - JOUR

T1 - Arbitrarily primed PCR fingerprinting of RNA

AU - Welsh, John

AU - Chada, Kiran

AU - Dalal, Seema S.

AU - Cheng, Rita

AU - Relph, David

AU - Mcclelland, Michael

N1 - Funding Information: We thank Rhonda Honeycutt and Bruno Sobral for critical reading of the manuscript. This work was supported by NTH grants 2R01HG00106, 1RO1HG00456 to MM, 1R29AI32644 to JW and 2RO1GM38731 to KC.

PY - 1992/10/11

Y1 - 1992/10/11

N2 - Fingerprinting of RNA populations was achieved using an arbitrarily selected primer at low stringency for first and second strand cDNA synthesis. PCR amplification was then used to amplify the products. The method required only a few nanograms of total RNA and was unaffected by low levels of genomlc double stranded DNA contamination. A reproducible pattern of ten to twenty clearly visible PCR products was obtained from any one tissue. Differences in PCR fingerprints were detected for RNAs from the same tissue isolated from different mouse strains and for RNAs from different tissues from the same mouse. The strain-specific differences revealed are probably due to sequence polymorphisms and should be useful for genetic mapping of genes. The tissue-specific differences revealed may be useful for studying differential gene expression. Examples of tissue-specific differences were cloned. Differential expression was confirmed for these products by Northern analysis and DNA sequencing uncovered two new tissue-specific messages. The method should be applicable to the detection of differences between RNA populations in a wide variety of situations.

AB - Fingerprinting of RNA populations was achieved using an arbitrarily selected primer at low stringency for first and second strand cDNA synthesis. PCR amplification was then used to amplify the products. The method required only a few nanograms of total RNA and was unaffected by low levels of genomlc double stranded DNA contamination. A reproducible pattern of ten to twenty clearly visible PCR products was obtained from any one tissue. Differences in PCR fingerprints were detected for RNAs from the same tissue isolated from different mouse strains and for RNAs from different tissues from the same mouse. The strain-specific differences revealed are probably due to sequence polymorphisms and should be useful for genetic mapping of genes. The tissue-specific differences revealed may be useful for studying differential gene expression. Examples of tissue-specific differences were cloned. Differential expression was confirmed for these products by Northern analysis and DNA sequencing uncovered two new tissue-specific messages. The method should be applicable to the detection of differences between RNA populations in a wide variety of situations.

UR - https://www.scopus.com/pages/publications/0026784966

UR - https://www.scopus.com/pages/publications/0026784966#tab=citedBy

U2 - 10.1093/nar/20.19.4965

DO - 10.1093/nar/20.19.4965

M3 - Article

C2 - 1383934

SN - 0305-1048

VL - 20

SP - 4965

EP - 4970

JO - Nucleic acids research

JF - Nucleic acids research

IS - 19

ER -