Artificial sequence-specific DNA binding peptides: Branched-chain basic regions

Matthew Pellegrini, Richard H. Ebright

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Branched-chain-basic-region peptides ('bcBR') consisting of two bZIP basic regions connected through a C-terminal N(α),N(ε)-lysine linkage bind to DNA with high affinity and high specificity. bcBR with two identical basic regions recognize 2-fold-symmetric DNA sites, and bcBR with two nonidentical basic regions recognize asymmetric DNA sites. Resin-linked bcBR ('bcBR beads'), which are compatible with one-bead/one-peptide combinatorial chemistry approaches, retain high-specificity DNA binding activity and permit high-throughput screening of DNA recognition properties.

Original languageEnglish (US)
Pages (from-to)5831-5835
Number of pages5
JournalJournal of the American Chemical Society
Volume118
Issue number25
DOIs
StatePublished - Jun 26 1996

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry
  • Catalysis
  • Colloid and Surface Chemistry

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