Assessing the abundance and polymorphism of simple sequence repeats in perennial rye grass

Christine Kubik, William A. Meyer, Brandon S. Gaut

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Simple sequence repeats (SSRs) have proven to be useful genetic markers in a wide variety of plants, but have yet to be widely applied to turfgrasses. Here we describe a study of SSRs in perennial ryegrass (Lolium perenne L.). A library of perennial ryegrass genomic DNA was screened with (GA)(n) and (GT)(n) probes, and SSR-containing clones were isolated and sequenced. On the basis of this screen, we estimated that there are roughly 5800 (GA)(n) and (GT)(n) SSRs in the haploid perennial ryegrass genome. Polymerase chain reaction (PCR) primers were designed to amplify the isolated SSRs, and six polymorphic SSRs were identified. Polymorphism in the these six SSRs was sufficient to discriminate among 18 individuals representing 11 perennial ryegrass clones and seven other Lolium species. Half-sibs could be distinguished with data from as few as three SSRs. The SSR genotype data was also used to infer genetic relationships among the individuals of our sample. The relationships were in broad agreement with those established by previous analyses, suggesting that SSR data will be useful for exploring relationships among perennial ryegrass cultivars. In total, this study indicates that SSRs are sufficiently abundant and sufficiently polymorphic to be useful genetic markers in perennial ryegrass.

Original languageEnglish (US)
Pages (from-to)1136-1141
Number of pages6
JournalCrop Science
Volume39
Issue number4
DOIs
StatePublished - 1999

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science

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