Attenuation of the protein wasting associated with bed rest by branched-chain amino acids

TY - JOUR

T1 - Attenuation of the protein wasting associated with bed rest by branched-chain amino acids

AU - Stein, T. Peter

AU - Schluter, Margaret D.

AU - Leskiw, Maria J.

AU - Boden, Guenther

N1 - Funding Information: This study was supported by NIH grants RO1 AG14078 (TPS), RO1 AG 07988 (GB), R01 AA 10221 (GB), and RR-349 (GB, GCRC branch of the NCRR).

PY - 1999/9

Y1 - 1999/9

N2 - Bed rest is generally accepted as being an appropriate ground-based model for human spaceflight. The objectives of this study were to test the hypothesis that increasing the amount of branched-chain amino acids (BCAAs) in the diet could attenuate the protein loss associated with bed rest. Nineteen healthy subjects were randomized into two groups according to diet. During the 6 d of bed rest, the diets were supplemented with either 30 mmol/d each of three non-essential amino acids, glycine, serine, and alanine (control group), or with 30 mmol/d each of the BCAAs, leucine, isoleucine, and valine (BCAA group). Nutrition was supplied as a commercially available defined formula diet at a rate of 1.3 x REE. Nitrogen (N) balance and urinary 3-MeH excretion were determined for the 6 d. In our results, the urine-based estimate of N balance was 22.2 ± 14.4 (n = 9) mg N · kg-1 . d-1 and 60.5 ± 10.1 mg (n = 8) N · kg-1 · d-1 for the control and BCAA-supplemented groups, respectively (P < 0.05). Urinary 3-MeH excretion was unchanged in both groups with bed rest. We conclude that BCAA supplementation attenuates the N loss during short-term bed rest. Copyright (C) 1999 Elsevier Science Inc.

AB - Bed rest is generally accepted as being an appropriate ground-based model for human spaceflight. The objectives of this study were to test the hypothesis that increasing the amount of branched-chain amino acids (BCAAs) in the diet could attenuate the protein loss associated with bed rest. Nineteen healthy subjects were randomized into two groups according to diet. During the 6 d of bed rest, the diets were supplemented with either 30 mmol/d each of three non-essential amino acids, glycine, serine, and alanine (control group), or with 30 mmol/d each of the BCAAs, leucine, isoleucine, and valine (BCAA group). Nutrition was supplied as a commercially available defined formula diet at a rate of 1.3 x REE. Nitrogen (N) balance and urinary 3-MeH excretion were determined for the 6 d. In our results, the urine-based estimate of N balance was 22.2 ± 14.4 (n = 9) mg N · kg-1 . d-1 and 60.5 ± 10.1 mg (n = 8) N · kg-1 · d-1 for the control and BCAA-supplemented groups, respectively (P < 0.05). Urinary 3-MeH excretion was unchanged in both groups with bed rest. We conclude that BCAA supplementation attenuates the N loss during short-term bed rest. Copyright (C) 1999 Elsevier Science Inc.

UR - https://www.scopus.com/pages/publications/0032819476

UR - https://www.scopus.com/pages/publications/0032819476#tab=citedBy

U2 - 10.1016/S0899-9007(99)00120-3

DO - 10.1016/S0899-9007(99)00120-3

M3 - Article

C2 - 10467608

SN - 0899-9007

VL - 15

SP - 656

EP - 660

JO - Nutrition

JF - Nutrition

IS - 9

ER -