Auxin boosts energy generation pathways to fuel pollen maturation in barley

Dhika Amanda; Felix P. Frey; Ulla Neumann; Marine Przybyl; Jan Šimura; Youjun Zhang; Zongliang Chen; Andrea Gallavotti; Alisdair R. Fernie; Karin Ljung; Iván F. Acosta

doi:10.1016/j.cub.2022.02.073

Auxin boosts energy generation pathways to fuel pollen maturation in barley

Dhika Amanda, Felix P. Frey, Ulla Neumann, Marine Przybyl, Jan Šimura, Youjun Zhang, Zongliang Chen, Andrea Gallavotti, Alisdair R. Fernie, Karin Ljung, Iván F. Acosta

Waksman Institute of Microbiology

Rutgers, The State University

Research output: Contribution to journal › Article › peer-review

Abstract

Pollen grains become increasingly independent of the mother plant as they reach maturity through poorly understood developmental programs. We report that the hormone auxin is essential during barley pollen maturation to boost the expression of genes encoding almost every step of heterotrophic energy production pathways. Accordingly, auxin is necessary for the flux of sucrose and hexoses into glycolysis and to increase the levels of pyruvate and two tricarboxylic (TCA) cycle metabolites (citrate and succinate). Moreover, bioactive auxin is synthesized by the pollen-localized enzyme HvYUCCA4, supporting that pollen grains autonomously produce auxin to stimulate a specific cellular output, energy generation, that fuels maturation processes such as starch accumulation. Our results demonstrate that auxin can shift central carbon metabolism to drive plant cell development, which suggests a direct mechanism for auxin's ability to promote growth and differentiation.

Original language	American English
Pages (from-to)	1798-1811.e8
Journal	Current Biology
Volume	32
Issue number	8
DOIs	https://doi.org/10.1016/j.cub.2022.02.073
State	Published - Apr 25 2022

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences

Keywords

anther
auxin
barley
metabolism
plant male fertility
pollen
stamen maturation
starch

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10.1016/j.cub.2022.02.073

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title = "Auxin boosts energy generation pathways to fuel pollen maturation in barley",

abstract = "Pollen grains become increasingly independent of the mother plant as they reach maturity through poorly understood developmental programs. We report that the hormone auxin is essential during barley pollen maturation to boost the expression of genes encoding almost every step of heterotrophic energy production pathways. Accordingly, auxin is necessary for the flux of sucrose and hexoses into glycolysis and to increase the levels of pyruvate and two tricarboxylic (TCA) cycle metabolites (citrate and succinate). Moreover, bioactive auxin is synthesized by the pollen-localized enzyme HvYUCCA4, supporting that pollen grains autonomously produce auxin to stimulate a specific cellular output, energy generation, that fuels maturation processes such as starch accumulation. Our results demonstrate that auxin can shift central carbon metabolism to drive plant cell development, which suggests a direct mechanism for auxin's ability to promote growth and differentiation.",

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author = "Dhika Amanda and Frey, {Felix P.} and Ulla Neumann and Marine Przybyl and Jan {\v S}imura and Youjun Zhang and Zongliang Chen and Andrea Gallavotti and Fernie, {Alisdair R.} and Karin Ljung and Acosta, {Iv{\'a}n F.}",

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doi = "10.1016/j.cub.2022.02.073",

language = "American English",

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T1 - Auxin boosts energy generation pathways to fuel pollen maturation in barley

AU - Amanda, Dhika

AU - Frey, Felix P.

AU - Neumann, Ulla

AU - Przybyl, Marine

AU - Šimura, Jan

AU - Zhang, Youjun

AU - Chen, Zongliang

AU - Gallavotti, Andrea

AU - Fernie, Alisdair R.

AU - Ljung, Karin

AU - Acosta, Iván F.

PY - 2022/4/25

Y1 - 2022/4/25

N2 - Pollen grains become increasingly independent of the mother plant as they reach maturity through poorly understood developmental programs. We report that the hormone auxin is essential during barley pollen maturation to boost the expression of genes encoding almost every step of heterotrophic energy production pathways. Accordingly, auxin is necessary for the flux of sucrose and hexoses into glycolysis and to increase the levels of pyruvate and two tricarboxylic (TCA) cycle metabolites (citrate and succinate). Moreover, bioactive auxin is synthesized by the pollen-localized enzyme HvYUCCA4, supporting that pollen grains autonomously produce auxin to stimulate a specific cellular output, energy generation, that fuels maturation processes such as starch accumulation. Our results demonstrate that auxin can shift central carbon metabolism to drive plant cell development, which suggests a direct mechanism for auxin's ability to promote growth and differentiation.

AB - Pollen grains become increasingly independent of the mother plant as they reach maturity through poorly understood developmental programs. We report that the hormone auxin is essential during barley pollen maturation to boost the expression of genes encoding almost every step of heterotrophic energy production pathways. Accordingly, auxin is necessary for the flux of sucrose and hexoses into glycolysis and to increase the levels of pyruvate and two tricarboxylic (TCA) cycle metabolites (citrate and succinate). Moreover, bioactive auxin is synthesized by the pollen-localized enzyme HvYUCCA4, supporting that pollen grains autonomously produce auxin to stimulate a specific cellular output, energy generation, that fuels maturation processes such as starch accumulation. Our results demonstrate that auxin can shift central carbon metabolism to drive plant cell development, which suggests a direct mechanism for auxin's ability to promote growth and differentiation.

KW - anther

KW - auxin

KW - barley

KW - metabolism

KW - plant male fertility

KW - pollen

KW - stamen maturation

KW - starch

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SN - 0960-9822

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SP - 1798-1811.e8

JO - Current Biology

JF - Current Biology

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ER -

Auxin boosts energy generation pathways to fuel pollen maturation in barley

Abstract

ASJC Scopus subject areas

Keywords

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