TY - JOUR
T1 - Bacillus amyloliquefaciens spore production under solid-state fermentation of lignocellulosic residues
AU - Berikashvili, Violet
AU - Sokhadze, Kakha
AU - Kachlishvili, Eva
AU - Elisashvili, Vladimir
AU - Chikindas, Michael L.
N1 - Funding Information: Funding Information This study was supported by the Shota Rustaveli National Science Foundation, Georgia, grant No. AR/106/7-280/14. Publisher Copyright: © Springer Science+Business Media, LLC, part of Springer Nature 2017.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - This study was conducted to elucidate cultivation conditions determining Bacillus amyloliquefaciens B-1895 growth and enhanced spore formation during the solid-state fermentation (SSF) of agro-industrial lignocellulosic biomasses. Among the tested growth substrates, corncobs provided the highest yield of spores (47 × 1010 spores g-1 biomass) while the mushroom spent substrate and sunflower oil mill appeared to be poor growth substrates for spore formation. Maximum spore yield (82 × 1010 spores g-1 biomass) was achieved when 15 g corncobs were moistened with 60 ml of the optimized nutrient medium containing 10 g peptone, 2 g KH2PO4, 1 g MgSO4·7H2O, and 1 g NaCl per 1 l of distilled water. The cheese whey usage for wetting of lignocellulosic substrate instead water promoted spore formation and increased the spore number to 105 × 1010 spores g-1. Addition to the cheese whey of optimized medium components favored sporulation process. The feasibility of developed medium and strategy was shown in scaled up SSF of corncobs in polypropylene bags since yield of 10 × 1011 spores per gram of dry biomass was achieved. In the SSF of lignocellulose, B. amyloliquefaciens B-1895 secreted comparatively high cellulase and xylanase activities to ensure good growth of the bacterial culture.
AB - This study was conducted to elucidate cultivation conditions determining Bacillus amyloliquefaciens B-1895 growth and enhanced spore formation during the solid-state fermentation (SSF) of agro-industrial lignocellulosic biomasses. Among the tested growth substrates, corncobs provided the highest yield of spores (47 × 1010 spores g-1 biomass) while the mushroom spent substrate and sunflower oil mill appeared to be poor growth substrates for spore formation. Maximum spore yield (82 × 1010 spores g-1 biomass) was achieved when 15 g corncobs were moistened with 60 ml of the optimized nutrient medium containing 10 g peptone, 2 g KH2PO4, 1 g MgSO4·7H2O, and 1 g NaCl per 1 l of distilled water. The cheese whey usage for wetting of lignocellulosic substrate instead water promoted spore formation and increased the spore number to 105 × 1010 spores g-1. Addition to the cheese whey of optimized medium components favored sporulation process. The feasibility of developed medium and strategy was shown in scaled up SSF of corncobs in polypropylene bags since yield of 10 × 1011 spores per gram of dry biomass was achieved. In the SSF of lignocellulose, B. amyloliquefaciens B-1895 secreted comparatively high cellulase and xylanase activities to ensure good growth of the bacterial culture.
KW - Bacillus amyloliquefaciens
KW - Cultivation conditions
KW - Lignocellulose solid-state fermentation
KW - Probiotic
KW - Spore production
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U2 - https://doi.org/10.1007/s12602-017-9371-x
DO - https://doi.org/10.1007/s12602-017-9371-x
M3 - Article
C2 - 29249066
VL - 10
SP - 755
EP - 761
JO - Probiotics and Antimicrobial Proteins
JF - Probiotics and Antimicrobial Proteins
SN - 1867-1306
IS - 4
ER -