Bacterial DNA is present in the fetal intestine and overlaps with that in the placenta in mice

Keith A. Martinez, Joann Romano-Keeler, Joseph P. Zackular, Daniel J. Moore, Robert M. Brucker, Christopher Hooper, Shufang Meng, Naoko Brown, Simon Mallal, Jeff Reese, David M. Aronoff, Hakdong Shin, Maria Gloria Dominguez-Bello, Jörn Hendrik Weitkamp

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Bacterial DNA has been reported in the placenta and amniotic fluid by several independent groups of investigators. However, it’s taxonomic overlap with fetal and maternal bacterial DNA in different sites has been poorly characterized. Here, we determined the presence of bacterial DNA in the intestines and placentas of fetal mice at gestational day 17 (n = 13). These were compared to newborn intestines (n = 15), maternal sites (mouth, n = 6; vagina, n = 6; colon, n = 7; feces, n = 8), and negative controls to rule out contamination. The V4 region of the bacterial 16S rRNA gene indicated a pattern of bacterial DNA in fetal intestine similar to placenta but with higher phylogenetic diversity than placenta or newborn intestine. Firmicutes were the most frequently assignable phylum. SourceTracker analysis suggested the placenta as the most commonly identifiable origin for fetal bacterial DNA, but also over 75% of fetal gut genera overlapped with maternal oral and vaginal taxa but not with maternal or newborn feces. These data provide evidence for the presence of bacterial DNA in the mouse fetus.

Original languageEnglish (US)
Article numbere0197439
JournalPLoS ONE
Volume13
Issue number5
DOIs
StatePublished - May 2018

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Bacterial DNA
Placenta
Intestines
Mothers
Feces
Vagina
Amniotic Fluid
rRNA Genes
Mouth
Colon
Fetus
Research Personnel

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Martinez, K. A., Romano-Keeler, J., Zackular, J. P., Moore, D. J., Brucker, R. M., Hooper, C., ... Weitkamp, J. H. (2018). Bacterial DNA is present in the fetal intestine and overlaps with that in the placenta in mice. PLoS ONE, 13(5), [e0197439]. https://doi.org/10.1371/journal.pone.0197439
Martinez, Keith A. ; Romano-Keeler, Joann ; Zackular, Joseph P. ; Moore, Daniel J. ; Brucker, Robert M. ; Hooper, Christopher ; Meng, Shufang ; Brown, Naoko ; Mallal, Simon ; Reese, Jeff ; Aronoff, David M. ; Shin, Hakdong ; Dominguez-Bello, Maria Gloria ; Weitkamp, Jörn Hendrik. / Bacterial DNA is present in the fetal intestine and overlaps with that in the placenta in mice. In: PLoS ONE. 2018 ; Vol. 13, No. 5.
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Martinez, KA, Romano-Keeler, J, Zackular, JP, Moore, DJ, Brucker, RM, Hooper, C, Meng, S, Brown, N, Mallal, S, Reese, J, Aronoff, DM, Shin, H, Dominguez-Bello, MG & Weitkamp, JH 2018, 'Bacterial DNA is present in the fetal intestine and overlaps with that in the placenta in mice', PLoS ONE, vol. 13, no. 5, e0197439. https://doi.org/10.1371/journal.pone.0197439

Bacterial DNA is present in the fetal intestine and overlaps with that in the placenta in mice. / Martinez, Keith A.; Romano-Keeler, Joann; Zackular, Joseph P.; Moore, Daniel J.; Brucker, Robert M.; Hooper, Christopher; Meng, Shufang; Brown, Naoko; Mallal, Simon; Reese, Jeff; Aronoff, David M.; Shin, Hakdong; Dominguez-Bello, Maria Gloria; Weitkamp, Jörn Hendrik.

In: PLoS ONE, Vol. 13, No. 5, e0197439, 05.2018.

Research output: Contribution to journalArticle

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AU - Weitkamp, Jörn Hendrik

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Martinez KA, Romano-Keeler J, Zackular JP, Moore DJ, Brucker RM, Hooper C et al. Bacterial DNA is present in the fetal intestine and overlaps with that in the placenta in mice. PLoS ONE. 2018 May;13(5). e0197439. https://doi.org/10.1371/journal.pone.0197439