Behavioral response of Lygus hesperus to conspecifics and headspace volatiles of alfalfa in a Y-tube olfactometer

J. L. Blackmer, Cesar Rodriguez-Saona, J. A. Byers, K. L. Shope, J. P. Smith

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

The western tarnished plant bug, Lygus hesperus Knight, feeds and develops on a variety of weeds in the spring, with later generations moving to alfalfa and cotton where severe damage to reproductive structures can occur. A synthetic attractant for monitoring or mass-trapping L. hesperus, or the identification of potential attractants for natural enemies, would be useful tools for integrated pest management programs. Studies investigated the response of naive and experienced fifth-instar and adult L. hesperus to odors associated with conspecifics and alfalfa, Medicago sativa L. Fifth-instar L. hesperus responded to all plant/insect combinations, whereas female L. hesperus only responded preferentially to vegetative and flowering alfalfa where conspecifics had fed for 24-72 hr, and to vegetative alfalfa where conspecifics were added approximately 30 min before the test began. Males were not attracted to headspace volatiles from any of the alfalfa treatments. Analysis of headspace volatiles showed that (E)-2-hexanal, (Z)-3-hexen-1-ol, α-pinene, (Z)-3-hexenyl acetate, (E)-2-hexenyl acetate, limonene, (Z)-ocimene, (E)-β-ocimene, linalool, (3E)-4,8-dimethyl-1,3,7-nonatriene, and (E, E)-α-farnesene are emitted from both vegetative and flowering alfalfa. Indole and (3E, 7E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene were only detected in flowering alfalfa. Damage to alfalfa by L. hesperus increased emissions of (Z)-ocimene, (E)-β-ocimene, (E)-β-caryophyllene, and (E, E)-α-farnesene, while β-pinene, myrcene, methyl salicylate, and (3E, 7E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene were only detected from damaged plants. Thus, individual or mixtures of these alfalfa volatiles may be useful as attractants for capturing nymphs and adult females of L. hesperus in the field.

Original languageEnglish (US)
Pages (from-to)1547-1564
Number of pages18
JournalJournal of Chemical Ecology
Volume30
Issue number8
DOIs
StatePublished - Aug 1 2004

Fingerprint

Lygus hesperus
Medicago sativa
olfactometers
behavioral response
alfalfa
headspace analysis
ocimene
Odors
Cotton
Acetates
attractant
attractants
flowering
Monitoring
farnesene
acetate
instars
acetates
4,8,12-trimethyl-1,3,7,11-tridecatetraene
methyl salicylate

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry

Keywords

  • Miridae
  • Y-tube olfactometer
  • alfalfa
  • herbivore-induced volatiles
  • host location
  • western tarnished plant bug

Cite this

@article{920441f5376245fd8a6ae9a22234a3c4,
title = "Behavioral response of Lygus hesperus to conspecifics and headspace volatiles of alfalfa in a Y-tube olfactometer",
abstract = "The western tarnished plant bug, Lygus hesperus Knight, feeds and develops on a variety of weeds in the spring, with later generations moving to alfalfa and cotton where severe damage to reproductive structures can occur. A synthetic attractant for monitoring or mass-trapping L. hesperus, or the identification of potential attractants for natural enemies, would be useful tools for integrated pest management programs. Studies investigated the response of naive and experienced fifth-instar and adult L. hesperus to odors associated with conspecifics and alfalfa, Medicago sativa L. Fifth-instar L. hesperus responded to all plant/insect combinations, whereas female L. hesperus only responded preferentially to vegetative and flowering alfalfa where conspecifics had fed for 24-72 hr, and to vegetative alfalfa where conspecifics were added approximately 30 min before the test began. Males were not attracted to headspace volatiles from any of the alfalfa treatments. Analysis of headspace volatiles showed that (E)-2-hexanal, (Z)-3-hexen-1-ol, α-pinene, (Z)-3-hexenyl acetate, (E)-2-hexenyl acetate, limonene, (Z)-ocimene, (E)-β-ocimene, linalool, (3E)-4,8-dimethyl-1,3,7-nonatriene, and (E, E)-α-farnesene are emitted from both vegetative and flowering alfalfa. Indole and (3E, 7E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene were only detected in flowering alfalfa. Damage to alfalfa by L. hesperus increased emissions of (Z)-ocimene, (E)-β-ocimene, (E)-β-caryophyllene, and (E, E)-α-farnesene, while β-pinene, myrcene, methyl salicylate, and (3E, 7E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene were only detected from damaged plants. Thus, individual or mixtures of these alfalfa volatiles may be useful as attractants for capturing nymphs and adult females of L. hesperus in the field.",
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Behavioral response of Lygus hesperus to conspecifics and headspace volatiles of alfalfa in a Y-tube olfactometer. / Blackmer, J. L.; Rodriguez-Saona, Cesar; Byers, J. A.; Shope, K. L.; Smith, J. P.

In: Journal of Chemical Ecology, Vol. 30, No. 8, 01.08.2004, p. 1547-1564.

Research output: Contribution to journalArticle

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AU - Rodriguez-Saona, Cesar

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KW - Miridae

KW - Y-tube olfactometer

KW - alfalfa

KW - herbivore-induced volatiles

KW - host location

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