Biological response to nonuniform distributions of ²¹⁰Po in multicellular clusters

TY - JOUR

T1 - Biological response to nonuniform distributions of 210Po in multicellular clusters

AU - Neti, Prasad V.S.V.

AU - Howell, Roger W.

PY - 2007/9

Y1 - 2007/9

N2 - Radionuclides are distributed nonuniformly in tissue. The present work examined the impact of nonuniformities at the multicellular level on the lethal effects of 110Po. A three-dimensional (3D) tissue culture model was used wherein V79 cells were labeled with 210Po-citrate and mixed with unlabeled cells, and multicellular clusters were formed by centrifugation. The labeled cells were located randomly in the cluster to achieve a uniform distribution of radioactivity at the macroscopic level that was nonuniform at the multicellular level. The clusters were maintained at 10.5°C for 72 h to allow α-particle decays to accumulate and then dismantled, and the cells were seeded for colony formation. Unlike typical survival curves for α particles, two-component exponential dose-response curves were observed for all three labeling conditions. Furthermore, the slopes of the survival curves for 100, 10 and 1% labeling were different. Neither the mean cluster absorbed dose nor a semi-empirical multicellular dosimetry approach could accurately predict the lethal effects of 210Po-citrate.

AB - Radionuclides are distributed nonuniformly in tissue. The present work examined the impact of nonuniformities at the multicellular level on the lethal effects of 110Po. A three-dimensional (3D) tissue culture model was used wherein V79 cells were labeled with 210Po-citrate and mixed with unlabeled cells, and multicellular clusters were formed by centrifugation. The labeled cells were located randomly in the cluster to achieve a uniform distribution of radioactivity at the macroscopic level that was nonuniform at the multicellular level. The clusters were maintained at 10.5°C for 72 h to allow α-particle decays to accumulate and then dismantled, and the cells were seeded for colony formation. Unlike typical survival curves for α particles, two-component exponential dose-response curves were observed for all three labeling conditions. Furthermore, the slopes of the survival curves for 100, 10 and 1% labeling were different. Neither the mean cluster absorbed dose nor a semi-empirical multicellular dosimetry approach could accurately predict the lethal effects of 210Po-citrate.

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U2 - https://doi.org/10.1667/RR0902.1

DO - https://doi.org/10.1667/RR0902.1

M3 - Article

C2 - 17705637

VL - 168

SP - 332

EP - 340

JO - Radiation Research

JF - Radiation Research

SN - 0033-7587

IS - 3

ER -