BMP-2 modulates expression of other growth factors in a rat fracture healing model

Jessica Ann Cottrell, Owen Keane, Sheldon Sutton Lin, James Patrick O'Connor

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Recombinant human bone morphogenetic protein-2 (rhBMP-2) is an osteo-inductive reagent that can be used therapeutically to promote spinal arthrodesis and fracture healing. Despite its clinical use, the effect of rhBMP-2 on expression of endogenous osteogenic growth factors is poorly described. The goal of this study was to determine if local rhBMP-2 treatment modulates expression of pro-osteogenic growth factors during fracture healing. Rat femur fractures were wrapped with control or rhBMP-2 collagen soaked sponge. Radiographic observations demon- strated that rhBMP-2 treatment enhanced fracture callus formation and decreased bridging time. Expression of fracture site growth factor mRNAs was determined by quantitative poly- merase chain reaction. The gene expression data demonstrated that rhBMP-2 treatment significantly increased mRNA levels of BMP-2 at day 4, BMP-4 at days 2, 4, 7, and 10, BMP-7 at day 2, and TNF-α at day 7. Treatment with rhBMP-2 was shown to significantly reduce mRNA levelsof BMP-2 onday2 and10,TNF-α on day 14,and BMP-6 ondays 2, 10, 14,and 21.TGFβ-1 was also significantly reduced on days 2 and 21. In conclusion, treatment with rhBMP-2 appears to enhance expression of other osteogenic factors that could contribute to bone formation.

Original languageEnglish (US)
Pages (from-to)127-135
Number of pages9
JournalJournal of Applied Biomedicine
Issue number3
StatePublished - Jul 1 2014

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)


  • Fracture healing
  • Growth factors
  • Rat RT-qPCR
  • Recombinant human bone morphogenetic protein-2


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