Cell-cell interactions are essential for maintenance of hepatocyte function in collagen gel but not on matrigel

Prabhas V. Moghe, Robin N. Coger, Mehmet Toner, Martin L. Yarmush

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

The objective of this study was to examine the importance of cellular aggregation for the maintenance of liver-specific functions in hepatocytes. We used two culture matrix systems (collagen sandwich and Matrigel) to examine the responsiveness of albumin secretory function in cultured rat hepatocytes under various seeding conditions. With high cell seeding, both culture systems elicited comparable levels of elevated function. Under conditions of sparse seeding, however, their responses were quite distinct: collagen sandwiched cells exhibited a significant deterioration in secretion, while Matrigel-cultured cells retained their basal levels of function. This indicates that a critical degree of cell-cell interactions is essential for promoting function in the collagen sandwich, and in the Matrigel-cultured cells functions may be preserved by constitutive matrix-related phenomena, even in the absence of aggregation.

Original languageEnglish (US)
Pages (from-to)706-711
Number of pages6
JournalBiotechnology and Bioengineering
Volume56
Issue number6
DOIs
StatePublished - Dec 20 1997

Fingerprint

Collagen
Cell Communication
Hepatocytes
Gels
Maintenance
Cultured Cells
Albumins
Agglomeration
Cells
Liver
Deterioration
matrigel
Rats

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Bioengineering
  • Biotechnology

Keywords

  • Aggregation
  • Cell seeding density
  • Collagen sandwich
  • Hepatocytes
  • Matrigel

Cite this

@article{315a191a8c2d467d91e976228360f697,
title = "Cell-cell interactions are essential for maintenance of hepatocyte function in collagen gel but not on matrigel",
abstract = "The objective of this study was to examine the importance of cellular aggregation for the maintenance of liver-specific functions in hepatocytes. We used two culture matrix systems (collagen sandwich and Matrigel) to examine the responsiveness of albumin secretory function in cultured rat hepatocytes under various seeding conditions. With high cell seeding, both culture systems elicited comparable levels of elevated function. Under conditions of sparse seeding, however, their responses were quite distinct: collagen sandwiched cells exhibited a significant deterioration in secretion, while Matrigel-cultured cells retained their basal levels of function. This indicates that a critical degree of cell-cell interactions is essential for promoting function in the collagen sandwich, and in the Matrigel-cultured cells functions may be preserved by constitutive matrix-related phenomena, even in the absence of aggregation.",
keywords = "Aggregation, Cell seeding density, Collagen sandwich, Hepatocytes, Matrigel",
author = "Moghe, {Prabhas V.} and Coger, {Robin N.} and Mehmet Toner and Yarmush, {Martin L.}",
year = "1997",
month = "12",
day = "20",
doi = "https://doi.org/10.1002/(SICI)1097-0290(19971220)56:6<706::AID-BIT14>3.0.CO;2-5",
language = "English (US)",
volume = "56",
pages = "706--711",
journal = "Biotechnology and Bioengineering",
issn = "0006-3592",
publisher = "Wiley-VCH Verlag",
number = "6",

}

Cell-cell interactions are essential for maintenance of hepatocyte function in collagen gel but not on matrigel. / Moghe, Prabhas V.; Coger, Robin N.; Toner, Mehmet; Yarmush, Martin L.

In: Biotechnology and Bioengineering, Vol. 56, No. 6, 20.12.1997, p. 706-711.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cell-cell interactions are essential for maintenance of hepatocyte function in collagen gel but not on matrigel

AU - Moghe, Prabhas V.

AU - Coger, Robin N.

AU - Toner, Mehmet

AU - Yarmush, Martin L.

PY - 1997/12/20

Y1 - 1997/12/20

N2 - The objective of this study was to examine the importance of cellular aggregation for the maintenance of liver-specific functions in hepatocytes. We used two culture matrix systems (collagen sandwich and Matrigel) to examine the responsiveness of albumin secretory function in cultured rat hepatocytes under various seeding conditions. With high cell seeding, both culture systems elicited comparable levels of elevated function. Under conditions of sparse seeding, however, their responses were quite distinct: collagen sandwiched cells exhibited a significant deterioration in secretion, while Matrigel-cultured cells retained their basal levels of function. This indicates that a critical degree of cell-cell interactions is essential for promoting function in the collagen sandwich, and in the Matrigel-cultured cells functions may be preserved by constitutive matrix-related phenomena, even in the absence of aggregation.

AB - The objective of this study was to examine the importance of cellular aggregation for the maintenance of liver-specific functions in hepatocytes. We used two culture matrix systems (collagen sandwich and Matrigel) to examine the responsiveness of albumin secretory function in cultured rat hepatocytes under various seeding conditions. With high cell seeding, both culture systems elicited comparable levels of elevated function. Under conditions of sparse seeding, however, their responses were quite distinct: collagen sandwiched cells exhibited a significant deterioration in secretion, while Matrigel-cultured cells retained their basal levels of function. This indicates that a critical degree of cell-cell interactions is essential for promoting function in the collagen sandwich, and in the Matrigel-cultured cells functions may be preserved by constitutive matrix-related phenomena, even in the absence of aggregation.

KW - Aggregation

KW - Cell seeding density

KW - Collagen sandwich

KW - Hepatocytes

KW - Matrigel

UR - http://www.scopus.com/inward/record.url?scp=0342437102&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0342437102&partnerID=8YFLogxK

U2 - https://doi.org/10.1002/(SICI)1097-0290(19971220)56:6<706::AID-BIT14>3.0.CO;2-5

DO - https://doi.org/10.1002/(SICI)1097-0290(19971220)56:6<706::AID-BIT14>3.0.CO;2-5

M3 - Article

C2 - 18642343

VL - 56

SP - 706

EP - 711

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

SN - 0006-3592

IS - 6

ER -