Cell fusion in tumor development and progression: Occurrence of cell fusion in primary methylcholanthrene‐induced tumorigenesis

M. B. Fortuna, M. J. Dewey, P. Furmanski

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Definitive evidence for the occurrence of cell fusion in tumorigenesis was sought in methylcholanthrene‐induced sarcomas. This was approached by using allophenic mice generated from strains differing for electrophoretic variants of the ubiquitous, dimeric enzyme glucose phosphate isomerase, with fusion assessed by heterodimer formation. Eighty‐three carefully trimmed primary tumor samples (from 23 individual tumors in allophenic mice) were analyzed, as were 1,140 clones derived from them. In all primary tumor samples, zymograms exhibited one GPI homopolymeric band. Expression of a hybrid band (indicative of a fusion event) was not observed in these samples. However, 9 (0.8%) of the tumor clones demonstrated a distinct and reproducible hybrid band which was uniformly lost upon recloning. Our data suggest that cell fusion, although uncommon, occurs in the clonogenic cell fraction during primary MCA tumorigenesis and is followed rapidly by chromosome segregation.

Original languageEnglish (US)
Pages (from-to)731-737
Number of pages7
JournalInternational Journal of Cancer
Volume44
Issue number4
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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