Cellular antioxidant activities of polyphenols isolated from Eucalyptus leaves (Eucalyptus grandis×Eucalyptus urophylla GL9)

Yunjiao Chen, Junjiang Wang, Yangwen Ou, Hongzhang Chen, Suyao Xiao, Guo Liu, Yong Cao, Qingrong Huang

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

An activity-guided isolation process was performed to identify the antioxidant constituents of Eucalyptus leaves ( Eucalyptus grandis×Eucalyptus urophylla GL9). The ethyl acetate extract showed the highest activity in scavenging DPPH radical among five different fractions extracted from 70% aqueous ethanol extract of Eucalyptus leaves. Nine known compounds possessing radical scavenging activity, which included gallic acid (1), quercetin-3-. O-β-d-glucuronide (Q-3-G-A) (2), ethyl gallate (3), tellimagrandin II(4), chlorogenic acid (5), 3-. O-galloyl-4,6-. O-[(S)-hexahydroxydiphenoyl]-. d-glucose (gemin D) (6), pedunculagin (7), tellimagrandin I (8), and 1,2,3,4,6-penta-. O-galloyl-. β-d-glucose (PGG) (9) were isolated and identified from the ethyl acetate extract. Our results indicated that ethyl acetate extract and hydrolysable tannins among nine compounds exhibited great antioxidant activity in both chemical-based (e.g. DPPH and ABTS assays) and cellular-based (i.e., Hep G2) antioxidant assays, and PGG showed the highest cellular antioxidant activity. This study suggested that ethyl acetate extract and the hydrolysable tannins in Eucalyptus leaves would be promising natural antioxidants in pharmaceutical, feed and food supplement industries.

Original languageEnglish (US)
Pages (from-to)737-745
Number of pages9
JournalJournal of Functional Foods
Volume7
Issue number1
DOIs
StatePublished - 2014

All Science Journal Classification (ASJC) codes

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Keywords

  • Cellular antioxidant activity
  • Eucalyptus leaves
  • Hydrolysable tannins

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