Cellular proliferation and telomerase activity in CHRF-288-11 cells

Xiao Yan Yang, Masayuki Kimura, Elisabeth Jeanclos, Abraham Aviv

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Telomerase activity is detected in many immortalized cell lines. Recent studies suggest that terminal differentiation of some of these cell lines is associated with a reduction in telomerase activity. However, the question remains whether the reduction in telomerase activity results from terminal differentiation or from cessation of cellular proliferation. This was explored in the megakaryocytic cell line CHRF-288-11. Cells were treated with phorbol 12-myristate 13-acetate (PMA), which induces terminal differentiation of CHRF-288-11 cells, EGTA, serum depletion, and okadaic acid. All treatments resulted in cessation of proliferation. Except for okadaic acid, these treatments also induced inhibition of telomerase within 7 days. Restoring the original growth conditions of cells treated with PMA, EGTA and serum depletion resulted in the reversal of telomerase inhibition and an acceleration of proliferation. Apparent inhibition of telomerase was observed to follow the cessation of proliferation, whereas enhanced telomerase activity was noted to precede acceleration in proliferation. Thus, telomerase activity usually reflects the proliferative status rather than the differentiated status of CHRF-288-11 cells.

Original languageEnglish (US)
Pages (from-to)1545-1555
Number of pages11
JournalLife Sciences
Volume66
Issue number16
DOIs
StatePublished - Mar 10 2000

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Keywords

  • CHRF- 288-11
  • Cell growth
  • Differentiation
  • Protein kinase C
  • Quiescence
  • Telomerase

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