Changes in binding of alpha interferon IFN-αA to HL-60 cells during myeloid differentiation

Jerome Langer, S. Pestka

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The differentiation of human leukemic HL-60 cells from their predominantly promyelocyte form to a neutrophil-like state can be induced by the addition of dimethylsulfoxide (DMSO) or retinoic acid (RA) to the growth medium. The binding of human recombinant interferon IFN-αA to the undifferentiated and differentiated HL-60 cells was investigated. Within 2 days after the addition of DMSO or retinoic acid to growing HL-60 cells, the binding of IFN-αA to treated cells increases significantly relative to its binding to untreated cells. The difference in binding of IFN-αA between the treated and untreated cells continues to increase for at least 3 days. Analysis of binding curves of IFN-αA to neutrophil-like and promyelocytic HL-60 cells leads to the conclusion that the increased binding of [125I]IFN-αA to neutrophil-like cells is primarily the result of an increase in the number of binding sites on these cells.

Original languageEnglish (US)
Pages (from-to)637-649
Number of pages13
JournalJournal of Interferon Research
Volume5
Issue number4
DOIs
StatePublished - Jan 1 1985
Externally publishedYes

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HL-60 Cells
Interferon-alpha
Neutrophils
Dimethyl Sulfoxide
Tretinoin
Granulocyte Precursor Cells
Interferons
Binding Sites
Growth

All Science Journal Classification (ASJC) codes

  • Virology
  • Immunology

Cite this

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abstract = "The differentiation of human leukemic HL-60 cells from their predominantly promyelocyte form to a neutrophil-like state can be induced by the addition of dimethylsulfoxide (DMSO) or retinoic acid (RA) to the growth medium. The binding of human recombinant interferon IFN-αA to the undifferentiated and differentiated HL-60 cells was investigated. Within 2 days after the addition of DMSO or retinoic acid to growing HL-60 cells, the binding of IFN-αA to treated cells increases significantly relative to its binding to untreated cells. The difference in binding of IFN-αA between the treated and untreated cells continues to increase for at least 3 days. Analysis of binding curves of IFN-αA to neutrophil-like and promyelocytic HL-60 cells leads to the conclusion that the increased binding of [125I]IFN-αA to neutrophil-like cells is primarily the result of an increase in the number of binding sites on these cells.",
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Changes in binding of alpha interferon IFN-αA to HL-60 cells during myeloid differentiation. / Langer, Jerome; Pestka, S.

In: Journal of Interferon Research, Vol. 5, No. 4, 01.01.1985, p. 637-649.

Research output: Contribution to journalArticle

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AU - Pestka, S.

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AB - The differentiation of human leukemic HL-60 cells from their predominantly promyelocyte form to a neutrophil-like state can be induced by the addition of dimethylsulfoxide (DMSO) or retinoic acid (RA) to the growth medium. The binding of human recombinant interferon IFN-αA to the undifferentiated and differentiated HL-60 cells was investigated. Within 2 days after the addition of DMSO or retinoic acid to growing HL-60 cells, the binding of IFN-αA to treated cells increases significantly relative to its binding to untreated cells. The difference in binding of IFN-αA between the treated and untreated cells continues to increase for at least 3 days. Analysis of binding curves of IFN-αA to neutrophil-like and promyelocytic HL-60 cells leads to the conclusion that the increased binding of [125I]IFN-αA to neutrophil-like cells is primarily the result of an increase in the number of binding sites on these cells.

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