Characterization and cloning of grape circular rnas identified the cold resistance-related vv-circats1

Zhen Gao, Jing Li, Meng Luo, Hui Li, Qiuju Chen, Lei Wang, Shiren Song, Liping Zhao, Wenping Xu, Caixi Zhang, Shiping Wang, Chao Ma

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


Circular RNAs (circRNAs) are widely distributed and play essential roles in a series of developmental processes, although none have been identified or characterized in grapevines (Vitis vinifera). In this study, we characterized the function of grape circRNA and uncovered thousands of putative back-splicing sites by global transcriptome analysis. Our results indicated that several reported circRNA prediction algorithms should be used simultaneously to obtain comprehensive and reliable circRNA predictions in plants. Furthermore, the length of introns flanking grape circRNAs was closely related to exon circularization. Although the longer introns flanking grape circRNAs appeared to circularize more efficiently, a 20- to 50-nt region seemed large enough to drive grape circRNA biogenesis. In addition, the endogenous introns flanking circularized exon(s) in conjunction with reverse complementary sequences could support the accurate and efficient circularization of various exons in grape, which constitutes a new tool for exploring the functional consequences caused by circRNA expression. Finally, we identified 475 differentially expressed circRNAs in grape leaves under cold stress. Overexpression of Vv-circATS1, a circRNA derived from glycerol-3-P acyltransferase, improved cold tolerance in Arabidopsis (Arabidopsis thaliana), while the linear RNA derived from the same sequence cannot.

Original languageAmerican English
Pages (from-to)966-985
Number of pages20
JournalPlant physiology
Issue number2
StatePublished - Jun 2019
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science


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