TY - JOUR
T1 - Characterization and cloning of grape circular rnas identified the cold resistance-related vv-circats1
AU - Gao, Zhen
AU - Li, Jing
AU - Luo, Meng
AU - Li, Hui
AU - Chen, Qiuju
AU - Wang, Lei
AU - Song, Shiren
AU - Zhao, Liping
AU - Xu, Wenping
AU - Zhang, Caixi
AU - Wang, Shiping
AU - Ma, Chao
N1 - Funding Information: 1This work was supported by the National Key Research and Development Program of China (grant no. 2018YFD1000300), the National Natural Science Foundation of China (grant no. 31701888), the Shanghai Natural Science Foundation (grant no. 17ZR1448800) and the Special Funds of Modern Industrial Technology System for Agriculture (CARS-29-zp-7). 2Author for contact: chaoma2015@sjtu.edu.cn 3Senior author. Publisher Copyright: © 2019 American Society of Plant Biologists. All Rights Reserved.
PY - 2019/6
Y1 - 2019/6
N2 - Circular RNAs (circRNAs) are widely distributed and play essential roles in a series of developmental processes, although none have been identified or characterized in grapevines (Vitis vinifera). In this study, we characterized the function of grape circRNA and uncovered thousands of putative back-splicing sites by global transcriptome analysis. Our results indicated that several reported circRNA prediction algorithms should be used simultaneously to obtain comprehensive and reliable circRNA predictions in plants. Furthermore, the length of introns flanking grape circRNAs was closely related to exon circularization. Although the longer introns flanking grape circRNAs appeared to circularize more efficiently, a 20- to 50-nt region seemed large enough to drive grape circRNA biogenesis. In addition, the endogenous introns flanking circularized exon(s) in conjunction with reverse complementary sequences could support the accurate and efficient circularization of various exons in grape, which constitutes a new tool for exploring the functional consequences caused by circRNA expression. Finally, we identified 475 differentially expressed circRNAs in grape leaves under cold stress. Overexpression of Vv-circATS1, a circRNA derived from glycerol-3-P acyltransferase, improved cold tolerance in Arabidopsis (Arabidopsis thaliana), while the linear RNA derived from the same sequence cannot.
AB - Circular RNAs (circRNAs) are widely distributed and play essential roles in a series of developmental processes, although none have been identified or characterized in grapevines (Vitis vinifera). In this study, we characterized the function of grape circRNA and uncovered thousands of putative back-splicing sites by global transcriptome analysis. Our results indicated that several reported circRNA prediction algorithms should be used simultaneously to obtain comprehensive and reliable circRNA predictions in plants. Furthermore, the length of introns flanking grape circRNAs was closely related to exon circularization. Although the longer introns flanking grape circRNAs appeared to circularize more efficiently, a 20- to 50-nt region seemed large enough to drive grape circRNA biogenesis. In addition, the endogenous introns flanking circularized exon(s) in conjunction with reverse complementary sequences could support the accurate and efficient circularization of various exons in grape, which constitutes a new tool for exploring the functional consequences caused by circRNA expression. Finally, we identified 475 differentially expressed circRNAs in grape leaves under cold stress. Overexpression of Vv-circATS1, a circRNA derived from glycerol-3-P acyltransferase, improved cold tolerance in Arabidopsis (Arabidopsis thaliana), while the linear RNA derived from the same sequence cannot.
UR - http://www.scopus.com/inward/record.url?scp=85067218736&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85067218736&partnerID=8YFLogxK
U2 - https://doi.org/10.1104/pp.18.01331
DO - https://doi.org/10.1104/pp.18.01331
M3 - Article
C2 - 30962290
SN - 0032-0889
VL - 180
SP - 966
EP - 985
JO - Plant physiology
JF - Plant physiology
IS - 2
ER -