Characterization of ruminal bacteria producing a toxin associated with a bovine paraplegic syndrome

María G. Domínguez-Bello; Milagros Lovera; Carlos Sevcik; Julio C. Brito

doi:10.1016/0041-0101(93)90343-H

Characterization of ruminal bacteria producing a toxin associated with a bovine paraplegic syndrome

María G. Domínguez-Bello, Milagros Lovera, Carlos Sevcik, Julio C. Brito

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Abstract

We studied the ruminal population densities of bacteria in animals with and without bovine paraplegic syndrome (BPS). All bacterial counts were performed under strict anaerobiosis. Although the rumen bacterial density was around 10⁹ bacteria/ml in animals, both apparently healthy or suffering from BPS, a shift towards Gram-negative strains occurred in animals with BPS. The toxin added to the cultures stimulated bacterial growth. Bacterial strains from the rumen could produce the toxin in vitro. Gram-positive bacteria differed in their ability to produce the toxin; Streptomyces bovis did not produce the toxin, while Lactobacillus vitulinum was an efficient producer. All Gram-negative bacteria tested could produce the compound.

Original language	American English
Pages (from-to)	1595-1600
Number of pages	6
Journal	Toxicon
Volume	31
Issue number	12
DOIs	https://doi.org/10.1016/0041-0101(93)90343-H
State	Published - Dec 1993
Externally published	Yes

ASJC Scopus subject areas

Toxicology

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10.1016/0041-0101(93)90343-H

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@article{92ef614c507a4e5787cd45f95babc06c,

title = "Characterization of ruminal bacteria producing a toxin associated with a bovine paraplegic syndrome",

abstract = "We studied the ruminal population densities of bacteria in animals with and without bovine paraplegic syndrome (BPS). All bacterial counts were performed under strict anaerobiosis. Although the rumen bacterial density was around 109 bacteria/ml in animals, both apparently healthy or suffering from BPS, a shift towards Gram-negative strains occurred in animals with BPS. The toxin added to the cultures stimulated bacterial growth. Bacterial strains from the rumen could produce the toxin in vitro. Gram-positive bacteria differed in their ability to produce the toxin; Streptomyces bovis did not produce the toxin, while Lactobacillus vitulinum was an efficient producer. All Gram-negative bacteria tested could produce the compound.",

author = "Dom{\'i}nguez-Bello, \{Mar{\'i}a G.\} and Milagros Lovera and Carlos Sevcik and Brito, \{Julio C.\}",

note = "Funding Information: Acknowledgements---The authors are indebted to Drs S. CGNT\textasciitilde{}.Po\textasciitilde{}S and N. MAnQUEZ-QUrVE\textasciitilde{}f or their assistance with the veterinary procedures required to take samples from cows. Ms DHUWVAO TERO-PALMAd rew the figures. This project was partially financed by the following Venezuelan programs and institutions: 'Programa de Cooperaci6n Agricola Convenio MAC/PDVSA Sindrome Parapl6jico del Bovino y mejoramiento integral de la Granaderia', and the Consejo Nacional de Investigaciones Cientificas y Tecnol6gicas (CONICIT) (Project SI-2086).",

year = "1993",

month = dec,

doi = "10.1016/0041-0101(93)90343-H",

language = "American English",

volume = "31",

pages = "1595--1600",

journal = "Toxicon",

issn = "0041-0101",

publisher = "Elsevier Ltd",

number = "12",

}

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T1 - Characterization of ruminal bacteria producing a toxin associated with a bovine paraplegic syndrome

AU - Domínguez-Bello, María G.

AU - Lovera, Milagros

AU - Sevcik, Carlos

AU - Brito, Julio C.

N1 - Funding Information: Acknowledgements---The authors are indebted to Drs S. CGNT~.Po~S and N. MAnQUEZ-QUrVE~f or their assistance with the veterinary procedures required to take samples from cows. Ms DHUWVAO TERO-PALMAd rew the figures. This project was partially financed by the following Venezuelan programs and institutions: 'Programa de Cooperaci6n Agricola Convenio MAC/PDVSA Sindrome Parapl6jico del Bovino y mejoramiento integral de la Granaderia', and the Consejo Nacional de Investigaciones Cientificas y Tecnol6gicas (CONICIT) (Project SI-2086).

PY - 1993/12

Y1 - 1993/12

N2 - We studied the ruminal population densities of bacteria in animals with and without bovine paraplegic syndrome (BPS). All bacterial counts were performed under strict anaerobiosis. Although the rumen bacterial density was around 109 bacteria/ml in animals, both apparently healthy or suffering from BPS, a shift towards Gram-negative strains occurred in animals with BPS. The toxin added to the cultures stimulated bacterial growth. Bacterial strains from the rumen could produce the toxin in vitro. Gram-positive bacteria differed in their ability to produce the toxin; Streptomyces bovis did not produce the toxin, while Lactobacillus vitulinum was an efficient producer. All Gram-negative bacteria tested could produce the compound.

AB - We studied the ruminal population densities of bacteria in animals with and without bovine paraplegic syndrome (BPS). All bacterial counts were performed under strict anaerobiosis. Although the rumen bacterial density was around 109 bacteria/ml in animals, both apparently healthy or suffering from BPS, a shift towards Gram-negative strains occurred in animals with BPS. The toxin added to the cultures stimulated bacterial growth. Bacterial strains from the rumen could produce the toxin in vitro. Gram-positive bacteria differed in their ability to produce the toxin; Streptomyces bovis did not produce the toxin, while Lactobacillus vitulinum was an efficient producer. All Gram-negative bacteria tested could produce the compound.

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JO - Toxicon

JF - Toxicon

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ER -

Characterization of ruminal bacteria producing a toxin associated with a bovine paraplegic syndrome

Abstract

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