Cloning of the D-lactate dehydrogenase gene from Leuconostoc mesenteroides subsp. cremoris

V. Phalip, V. Dartois, P. Schmitt, C. Divies

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

A genomic library from Leuconostoc mesenteroides subsp. cremoris was screened for D-lactate dehydrogenase activity using a stereospecific lactate detection test on agar plate. Among 3500 clones tested, six positive colonies were found on D-lactate detection plate, displaying significantly higher D-LDH activity than Escherichia coli host strain.

Original languageAmerican English
Pages (from-to)221-226
Number of pages6
JournalBiotechnology Letters
Volume16
Issue number3
DOIs
StatePublished - Mar 1994
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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